| Lu Hongda,Dong Kechen,Lei Zhang,Lu Chi,Chen Weiqun,Kong Qingzhi.The protection of lianhuaqingwen against acute radiation-induced lung injury in rats[J].Chinese Journal of Radiological Medicine and Protection,2014,34(6):419-422 |
| The protection of lianhuaqingwen against acute radiation-induced lung injury in rats |
| Received:July 04, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.06.005 |
| KeyWords:Acute radiation-induced lung injury Lianhuaqingwen capsule Tumor necrosis factor-α (TNF-α) Interleukin-6 (IL-6) Protection |
| FundProject:国家自然科学基金（81372931，81101550）；湖北省自然科学基金（2012FFB05904）；武汉市科技攻关计划（2013060602010253） |
| Author Name | Affiliation | E-mail | | Lu Hongda | Department of Oncology, Wuhan Central Hospital, Wuhan 430014, China | | | Dong Kechen | 湖北中医药大学临床医学院 | | | Lei Zhang | Department of Oncology, Wuhan Central Hospital, Wuhan 430014, China | | | Lu Chi | Department of Oncology, Wuhan Central Hospital, Wuhan 430014, China | | | Chen Weiqun | 武汉市肿瘤研究所 | | | Kong Qingzhi | Department of Oncology, Wuhan Central Hospital, Wuhan 430014, China | kqzswz@hotmail.com |
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| Abstract:: |
| Objective To investigate the radioprotective function of lianhuaqingwen (LHQW) in rat acute radiation-induced lung injury. Methods Totally 36 female Wistar rats were randomized into 3 groups as administered group (treated by LHQW plus radiation), radiation group irradiated with a single of 20 Gy in 6 MV X-ray by Elekta Synergy VMAT, and blank control group without radiation. Performance status (PS) was estimated during 31 d of LHQW instragastric administration. After rats being sacrificed at 1, 14, 28 d of LHQW adminstration, the pathomorphological changes were observed in trauma lung tissue, the cell number in BALF (Bronchoalveolar lavage fluid) was counted, the levels of TNF-α and IL-6 in serum were measured by ELISA, and TNF-α and IL-6 mRNA expressions in lung tissue were assayed by RT-PCR. Results After LHQW treatment, the PS of rat was significantly elevated with less inflammation in morphous, and the cell number in BALF was markedly decreased in compare with radiation alone group. Furthermore, the serum levels of TNF-α and IL-6 were obviously reduced (tTNF-α=7.372,2.891,tIL-6=6.335,3.257,P <0.05) and the TNF-α and IL-6 mRNA levels in lung tissue were also decreased (tTNF-αmRNA=3.714,2.144,tIL-6 mRNA=3.589,2.883,P<0.05). Conclusions LHQW plays a protective role against acute radiation-induced lung injury in rats and the down-expressions of TNF-α and IL-6 may be involved. |
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