| Du Lifa,Liu Jingjia,Huang Li,Zhao Yong,Wang Junjie.The inhibition effects of continuous low dose rate radiation of 125I radioactive seeds on KYSE150 esophageal cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2014,34(6):415-418 |
| The inhibition effects of continuous low dose rate radiation of 125I radioactive seeds on KYSE150 esophageal cancer cells |
| Received:December 11, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.06.004 |
| KeyWords:Relative biological effectiveness Cell apoptosis G2/M cell cycle arrest Continuous low dose rate radiation |
| FundProject:教育部高等学校博士学科点专项科研基金（20120001110037） |
| Author Name | Affiliation | E-mail | | Du Lifa | Cancer Center, Peking University Third Hospital, Beijing 100191, China | | | Liu Jingjia | Cancer Center, Peking University Third Hospital, Beijing 100191, China | | | Huang Li | Cancer Center, Peking University Third Hospital, Beijing 100191, China | | | Zhao Yong | 中国科学院动物研究所 生物膜与膜生物工程国家重点实验室 | | | Wang Junjie | Cancer Center, Peking University Third Hospital, Beijing 100191, China | junjiewang_edu@sina.cn |
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| Abstract:: |
| Objective To determine the biological effectiveness of 125I radioactive seeds with continuous low dose rate radiation on the human esophageal cancer cell line KYSE150 in vitro and explore the underlying cellular mechanisms. Methods The cells were divided into three cell groups: control group, single dose radiation group (SDR) and 125I radioactive seeds with continuous low dose rate radiation group (125I-CLDR). The KYSE150 cells were exposed to radiation of X-ray at a high dose rate of 1.052 Gy/min or 125I radioactive seeds at a low dose rate of 2.77 cGy/h. The responses of KYSE150 cells to two modes of irradiation were evaluated by the colony-forming assay, cell apoptosis as well as cell cycle analysis. Furthermore, the expression levels of γ-H2AX and Bax were detected by Western blot. Results KYSE150 cells were more radiosensitive to 125I-CLDR than SDR. The relative biological effectiveness (RBE) for 125I-CLDR related to SDR was 1.56. Compared with SDR, 125I-CLDR yielded more proportions of the early and late apoptosis rate (t=4.07, 11.08, P<0.05) as well as cells at G2/M phase (t=11.25, P<0.05). Moreover, γ-H2AX and Bax expression levels in 125I-CLDR significantly increased compared with SDR. Conclusions Compared with the high dose rate X-ray radiation, the continuous low dose rate radiation of 125I radioactive seeds had stronger inhibition effect on KYSE150 esophageal cancer cells by impairing clonogenic capacity, inducing apoptosis and G2/M cell cycle arrest, and increasing radiosensitivity. |
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