| Yu Chenxiao,Duan Weiming,Zhou Xinwen.Mechanism of the radioresistant effect of LyGDI on NSCLC A549 cells[J].Chinese Journal of Radiological Medicine and Protection,2014,34(6):411-414 |
| Mechanism of the radioresistant effect of LyGDI on NSCLC A549 cells |
| Received:July 12, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.06.003 |
| KeyWords:LyGDI Non-small-cell lung cancer A549 cell Radiation resistance |
| FundProject:国家自然科学基金（30570548，81071878） |
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| Abstract:: |
| Objective To elucidate the mechanism of radiation resistant effect of LyGDI on NSCLC A549 cells. Methods A549 and H460 cells were irradiated with X-rays of 0, 2, 4 and 6 Gy. The clone-forming assay was used to detect cell survival and radiosensitivity. The expressions of LyGDI and COX-2 (Cyclooxygenase-2), a key radiosensitivity-related protein, were detected using Western blot. The miR-34 families were analyzed with RT-PCR. 50 nmol/L mature miR-34c was transfected into A549 cells. Results The expression levels of LyGDI and COX-2 were much higher in radioresistive A549 cells than that in H460 cells. While the expression of miR-34a was quite low and miR-34b/c was hardly found in both NSCLC cells. Transfection of miR-34c into A549 cells strongly enhanced X-ray induced apoptosis by inhibiting the activations of LyGDI, COX-2, Bcl-2 and p21. Conclusions Up-regulation of LyGDI could induce COX-2 expression. The low expression of miR-34 family might be responsible for the radiation resistance of NSCLC cells. |
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