| Nie Liangqin,Zhou Juying,Wang Lili,et al.Radiosensitization effects of celecoxib on glioblastoma：comparison with oligodendrocyte cell line[J].Chinese Journal of Radiological Medicine and Protection,2014,34(5):342-344,375 |
| Radiosensitization effects of celecoxib on glioblastoma：comparison with oligodendrocyte cell line |
| Received:August 14, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.05.006 |
| KeyWords:Cox-2 inhibitor Celecoxib Radiosensitivity Glioblastoma |
| FundProject:国家自然科学基金青年科学基金（81302384） |
| Author Name | Affiliation | E-mail | | Nie Liangqin | Department of Radiation Oncology, First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | Zhou Juying | Department of Radiation Oncology, First Affiliated Hospital of Soochow University, Suzhou 215006, China | zhjuying@sohu.com | | Wang Lili | Department of Radiation Oncology, First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | Xu Xiaoting | Department of Radiation Oncology, First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | Qin Songbing | Department of Radiation Oncology, First Affiliated Hospital of Soochow University, Suzhou 215006, China | |
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| Abstract:: |
| Objective To compare the radiosensitivity effect of celecoxib on oln93 and u373 cells, and to explore the related mechanism.Methods Both oln93 cells and u373 cells were respectively divided into control group, drug group, radiation group and combined group when treated with celecoxib and irradiation.Cell survival ratio was evaluated by MTT assay and clogenic assay.Flow cytometry and Western blot assay were used to measure cell cycle and protein expression.Results Celecoxib had a similar effect on oln93 and u373 cells in enhancing the radiosensitivity(t=2.215-30.996,P<0.05;t=0.383-11.732,P<0.05)and blocking cell cycle in G0/G1(t=-6.1-5.141,P<0.05).Compared with the radiation group, the combined group showed S phase arrest(t=-18.174,P<0.05), and increase of Cyclin A protein(t=-8.087,P<0.05)in oln93 cells, and G2/M arrest and decrease of Cyclin B1 and DNA-PKcs and MRE11 protein(t=-8.838-10.45,P<0.05) in u373 cells.Conclusions Celecoxib illustrates a more sensitive radiosensitivity to u373 cells by regulating its cell cycle and DNA damage repair. |
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