| Chang Pengyu,Jiang Xin,Qu Chao,et al.Therapeutic effects of adipose-derived stem cells on healing intestinal epithelium after abdominal irradiation[J].Chinese Journal of Radiological Medicine and Protection,2014,34(4):271-274 |
| Therapeutic effects of adipose-derived stem cells on healing intestinal epithelium after abdominal irradiation |
| Received:July 12, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.04.008 |
| KeyWords:Adipose-derived stem cells Radiation-induced intestinal injury Restoration |
| FundProject:国家自然科学基金（81372929） |
| Author Name | Affiliation | E-mail | | Chang Pengyu | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Jiang Xin | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Qu Chao | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Luo Jinghua | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Jiang Xinping | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Dong Lihua | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Cui Shuang | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | | | Qu Yaqin | Department of Radiation Oncology, First Bethune Hospital of Jilin University, Changchun 130021, China | Quyaqin52@163.com |
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| Abstract:: |
| Objective To evaluate the therapeutic effect of human adipose-derived stem cells (ADSCs) on healing intestinal epithelium after abdominal irradiation. Methods A total of 40 Sprague-Dawley rats were irradiated to the whole abdomen with a single dose of 15 Gy. 17 rats were randomly selected and infused intraperitoneally with 5×106 Passage 6 ADSCs,and 17 rats that received abdominal irradiation alone were set as control, 6 rats were set as normal control. At 10 d post-irradiation, 3 rats from each group were randomly selected and terminated. Freshly isolated small intestinal tissues were used for pathological analysis and EGF, KGF, E-cadherin, Bcl-2 and BAX mRNA levels analysis, and life-span and survival of rats were also analyzed. Results Compared to the control, infusion of ADSCs maintained the integrity of irradiated epithelium with more proliferative epithelial cells(t=18.30,P<0.05)and less apoptotic cells(t=－32.48,P<0.05). Besides, the rats receiving ADSCs-infusion survived longer than the control(t=5.99,P<0.05). Conclusions ADSCs can have therapeutic effect on healing radiation-induced intestinal epithelium injury. |
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