| Yang Lina,Ran Juntao,Zhang Hong,Liu Yuanyuan,Sun Chao,Zhang Qiuning,Wang Xinyu,Wang Xiaohu.Biological effects of heavy ion and X-ray irradiation on lung cancer cells A549[J].Chinese Journal of Radiological Medicine and Protection,2014,34(4):263-266 |
| Biological effects of heavy ion and X-ray irradiation on lung cancer cells A549 |
| Received:August 28, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.04.006 |
| KeyWords:Heavy ions X-rays Apoptosis DNA-PKcs |
| FundProject:国家重点基础研究发展计划（2010CB834202）；国家自然科学基金（81160283） |
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| Abstract:: |
| Objective To compare the effects of carbon heavy ion and X-ray irradiation on survival fraction, cell cycle, cell apoptosis and expression of DNA-PKcs of A549 lung cancer cells. Methods A549 cells were irradiated by carbon heavy ion and X-ray. Cell survival fraction, cell cycle and apoptosis were analyzed by clonogenic formation assay, flow cytometry and Hoechst 33258 staining, respectively. Real time-PCR was performed to detect the expressions of DNA-PKcs and H2AX mRNA. Results Lower cell survival fraction, more G2/M phase arrest and higher apoptosis rate were detected in the A549 cells exposed to carbon heavy ion than X-ray(t=4.77,14.53,14.54,P<0.05). Expression of DNA-PKcs was up-regulated after irradiation to carbon heavy ion and X-ray(t=10.91,5.05,P<0.05). Conclusions Both heavy ion and X-ray irradiations enhance the expression of DNA-PKcs, induce apoptosis through regulating cell cycle arrest, and hence reduce survival of A549 cells. Heavy ion irradiation shows more stronger biological effects than X-ray irradiation. |
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