| Zhao Hua,Lu Xue,Chen Deqing,et al.Dose-effect of nucleoplasmic bridges frequencies in human peripheral blood lymphocytes induced by 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2014,34(4):259-262 |
| Dose-effect of nucleoplasmic bridges frequencies in human peripheral blood lymphocytes induced by 60Co γ-rays |
| Received:September 05, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.04.005 |
| KeyWords:Ionizing radiation Nucleoplasmic bridge Micronucleus Dose response |
| FundProject:国家自然科学基金（30870749，81172593） |
| Author Name | Affiliation | E-mail | | Zhao Hua | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Chen Deqing | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | qjliu@nirp.cn |
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| Abstract:: |
| Objective To establish the analysis criteria of nucleoplasmic bridges (NPB) and a dose-response curve of NPB frequencies in human peripheral blood lymphocytes irradiated by 60Coγ-rays. Methods Human peripheral blood samples were collected from three healthy males, and were irradiated with 0,1, 2, 3, 4, 5 and 6 Gy of 60Coγ-rays. A cytokinesis-block micronucleus assay was carried out to analyze NPBs and micronuclei (MN) in binucleated cells. Results NPBs in binucleated cells at each dose level of γ-ray was conformed to the Poisson distribution. The dose-response curve of the γ-ray-induced NPB frequencies in human peripheral lymphocytes followed the linear-quadratic model y=(1.39×10-3)x2 + (4.94×10-3)x (R2=0.981, P < 0.05). Conclusions The dose-response curve of NPB frequencies in human peripheral blood lymphocytes induced by 0-6 Gy 60Coγ-rays was established. |
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