| He Wei,Zhou Fuxiang,Xu Hui,Jiang Huangang,Hu Liu,Xie Conghua,Zhou Yunfeng.Establishment of mitochondrial DNA depleted A549 cell line and the change of its radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2014,34(4):255-258 |
| Establishment of mitochondrial DNA depleted A549 cell line and the change of its radiosensitivity |
| Received:July 14, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.04.004 |
| KeyWords:Mitochondrial DNA deletion ρ0 cell mtDNA A549 Radiosensitivity |
| FundProject:国家自然科学基金（81172129） |
| Author Name | Affiliation | E-mail | | He Wei | Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biology Behavior of Hubei Province, Wuhan 430071, China | | | Zhou Fuxiang | Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biology Behavior of Hubei Province, Wuhan 430071, China | happyzhoufx@sina.com | | Xu Hui | Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biology Behavior of Hubei Province, Wuhan 430071, China | | | Jiang Huangang | Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biology Behavior of Hubei Province, Wuhan 430071, China | | | Hu Liu | Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biology Behavior of Hubei Province, Wuhan 430071, China | | | Xie Conghua | Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biology Behavior of Hubei Province, Wuhan 430071, China | | | Zhou Yunfeng | Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biology Behavior of Hubei Province, Wuhan 430071, China | |
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| Abstract:: |
| Objective To establish the mitochondrial DNA depleted cell line (ρ0 cells) of lung cancer cell A549 and to observe the radiosensitivity difference between ρ0 cells and normal A549 cells (ρ+ cells). Methods The ρ0 cells were depleted of mitochondrial DNA by culturing chronically in the presence of low concentration of ethidium bromide (EB), and then the cell model was confined.Radiosensitivity of both ρ0 cells and ρ+ cells was detected using the cologenic formation assay. After 6 MV X-ray irradiation in vitro, cell cycle distribution and reactive oxygen species (ROS) level were analyzed by flow cytometry and fluorescence microplate reader, respectively. Results A ρ0 cell line was successfully established and had a lower radiosensitivity than ρ+ cells (t=12.57, P<0.01). After irradiation with a dose of 8 Gy, compared to ρ+ cells, ρ0 cells showed prolonged G2 arrest with less cells in G2 (t=6.82, P<0.01) and had lower increase of ROS level (t=14.51, P<0.01). Conclusions ρ0 cells have a lower radiosensitivity than ρ+ cells, in which the reduction of ROS production and the prolongation of G2 arrest post-irradiation may be involved. |
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