| Wang Jinfeng,Sun Xinchen,Kang Yahui,et al.Mechanism of the prevention effect of a free radical scavenger, Tempol, on radiation-induced dysfunction in the submandibular gland of mouse[J].Chinese Journal of Radiological Medicine and Protection,2014,34(3):176-179 |
| Mechanism of the prevention effect of a free radical scavenger, Tempol, on radiation-induced dysfunction in the submandibular gland of mouse |
| Received:June 09, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.03.005 |
| KeyWords:Radiation injury Tempol Oxidative stress Submandibular gland |
| FundProject: |
| Author Name | Affiliation | E-mail | | Wang Jinfeng | Department of Radiation Oncology, The Affiliated Lianyungang Hospital of Bengbu Medical College, Lianyungang 222000, China | | | Sun Xinchen | 江苏省人民医院放疗科 | | | Kang Yahui | Department of Radiation Oncology, The Affiliated Lianyungang Hospital of Bengbu Medical College, Lianyungang 222000, China | | | Wang Zhongmin | Department of Radiation Oncology, The Affiliated Lianyungang Hospital of Bengbu Medical College, Lianyungang 222000, China | | | Yang Xi | 江苏省人民医院放疗科 | | | Cai Jing | 南通市肿瘤医院放化疗科 | | | Zhang Qu | Department of Radiation Oncology, The Affiliated Lianyungang Hospital of Bengbu Medical College, Lianyungang 222000, China | | | He Xiangfeng | 南通市肿瘤医院放化疗科 | | | Ma Jianxin | Department of Radiation Oncology, The Affiliated Lianyungang Hospital of Bengbu Medical College, Lianyungang 222000, China | | | Huang Guanhong | Department of Radiation Oncology, The Affiliated Lianyungang Hospital of Bengbu Medical College, Lianyungang 222000, China | 15996587143@163.com |
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| Abstract:: |
| Objective To explore the possible mechanism of the prevention effect of Tempol (TPL) on radiation-induced submandibular gland dysfunction using a C57bl/6 mouse model. Methods One-hundred twenty male C57bl/6 mice were randomly divided into six groups: blank control group, radiation group, 25 mg/kg TPL group, 200 mg/kg TPL group, 25 mg/kg TPL+ radiation group and 200 mg/kg TPL+ radiation group. 25 mg/kg and 200 mg/kg TPL was injected to mice enterocoelia 10 min before 15 Gy X-ray radiation. Survival rate and weight change of the mice were observed 30 d after radiation. Salivary flow rate and mandibular gland apoptosis were detected at 3 and 30 d after radiation. Total superoxide dismutase activity, total glutathione and malondialdehyde content and Bcl-2, Bax, Caspase 3 expression levels were detected at 3 d after radiation. Results Salivary flow rates at 3 and 30 days in the radiation group were 5.67±1.05 and 5.27±1.34, lower than that of 200 mg/kg TPL + radiation group (13.12±1.17,13.09±2.71) significantly (F=226.4, 215.3, P<0.05) but had no significant difference (P>0.05) compared with 25 mg/kg TPL + radiation group (6.44±1.09,5.48±1.79). Pretreatment of mice with 200 mg/kg TPL could reduce radiation induced apoptosis and the content of MDA in tissue but increased the GSH content. The TPL also increased the activity of SOD, the expression of Bcl-2 protein level, but reduced the levels of Bax and cleaved Caspase-3 protein. Conclusions 200 mg/kg TPL could protect C57bl/6 mice salivary glands from radiation injury probably by alleviating oxidative stress and regulating the mitochondrial pathway of apoptosis except 25 mg/kg TPL. |
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