| Meng Daquan,Tang Qiu,Long Zhixiong,et al.Study of diphenylene iodonium in the prevention of radiation-induced lung injury[J].Chinese Journal of Radiological Medicine and Protection,2014,34(3):168-171 |
| Study of diphenylene iodonium in the prevention of radiation-induced lung injury |
| Received:April 26, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.03.003 |
| KeyWords:Diphenylene iodonium Radiation-induced lung injury Rats |
| FundProject:国家自然科学基金（30770648） |
| Author Name | Affiliation | E-mail | | Meng Daquan | Department of Respiratory Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | Tang Qiu | 武汉市中心医院肿瘤科 | | | Long Zhixiong | 武汉市第五医院肿瘤科 | | | Xin Jianbao | Department of Respiratory Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | Ye Hong | 华中科技大学同济医学院病理生理系 | | | Ma Wanli | Department of Respiratory Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | whmawl@aliyun.com |
|
| Hits: 4162 |
| Download times: 2806 |
| Abstract:: |
| Objective To investigate the mechanism of an inhibitor of NADPH oxidases, diphenylene iodonium (DPI), in preventing radiation-induced lung injury. Methods Totally 48 adult SD male rats were randomly classified into 4 groups: control group (C), radiation group (R), radiation plus DPI group (R+D) and DPI group (D). The radiation induced pulmonary injury model was preformed by using 6 MV X-rays to deliver 8 Gy per day for 5 consecutive days with 40 Gy in total to the thorax of each animal. Rats in R+D group were subcutaneously administered with 0.02% DPI (1 mg/kg) at 1 h prior to radiation while rats in D group received the same dose of DPI without radiation. DPI was given from 3 d before radiation to 30 d after the first radiation. Rats in C and D groups received the same dose of saline. Animals were sacrificed at 1 month and 6 months after radiation, respectively. The lungs were removed and processed for HE and Masson staining, hydroxyproline content measurement, and TGF-β1 immunohistochemical detection.Results At 1 month post-radiation, rats in R group showed typical alveolitis, the level of hydroxyproline was (0.69±0.05) μg/mg, and the positive area of TGF-β1 expression was (39.97±0.90)%, while the level of hydroxyproline in R+D group was (0.55±0.03) μg/mg and the positive area of TGF-β1 expression was(33.83±1.55)%, rats in R+D group showed less severe alveolitis compared with R group(t=5.32,5.93,P<0.05). At 6 months post-radiation, rats in R group showed typical lung fibrosis with hydroxyproline level of (1.04±0.02) μg/mg and TGF-β1 expression of (37.80±0.85)%, whereas the hydroxyproline level in R+D group was (0.85±0.02) μg/mg, the TGF-β1 expression was(23.93±1.16)%, rats in R+D group showed moderate lung fibrosis(t=15.77,16.68,P<0.05), rats in C and D group had no noticeable changes.Conclusions Diphenylene iodonium could prevent radiation-induced lung injury by reducing the level of hydroxyproline and the expression of TGF-β1. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
