| Li Lili,Wang Qin,Hao Jianxiu,et al.The protective effect of 2,2-dimethylthiazolidine on human peripheral blood lymphocytes in radiation-induced genetic damage[J].Chinese Journal of Radiological Medicine and Protection,2014,34(2):95-98 |
| The protective effect of 2,2-dimethylthiazolidine on human peripheral blood lymphocytes in radiation-induced genetic damage |
| Received:June 06, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.02.005 |
| KeyWords:2,2-dimethylthiazolidine Chromosome aberration Micronucleus Single cell gel electrophoresis Radioprotection |
| FundProject:卫生部行业科研专项（201002009） |
| Author Name | Affiliation | E-mail | | Li Lili | Tianjin Key Laboratory of Molecular Nuclear Medicine, Clinical Laborary, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | Wang Qin | Tianjin Key Laboratory of Molecular Nuclear Medicine, Clinical Laborary, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | Hao Jianxiu | Tianjin Key Laboratory of Molecular Nuclear Medicine, Clinical Laborary, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | Jiang Liping | Tianjin Key Laboratory of Molecular Nuclear Medicine, Clinical Laborary, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | Xu Wenqing | Tianjin Key Laboratory of Molecular Nuclear Medicine, Clinical Laborary, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | Jiang Enhai | Tianjin Key Laboratory of Molecular Nuclear Medicine, Clinical Laborary, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | Xing Zhiwei | Tianjin Key Laboratory of Molecular Nuclear Medicine, Clinical Laborary, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | Li Jin | Tianjin Key Laboratory of Molecular Nuclear Medicine, Clinical Laborary, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | ljfss222@sina.com |
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| Abstract:: |
| Objective To investigate the protective effect of 2,2-dimethylthiazolidine (KFL) on human peripheral blood lymphocytes in radiation-induced genetic damage. Methods Peripheral blood samples were collected from healthy volunteers and divided into normal control group, irradiation group and KFL protective groups with four different concentrations (0.2, 0.5, 1, 2 mmol/L). KFL was administrated 30 min before 2 Gy irradiation. Chromosome aberration analysis, micronucleus test and single cell gel electrophoresis were used to observe the protective effects of KFL on the radiation-induced genetic damage in human peripheral blood lymphocytes. Results Compared with the irradiation group, the four KFL protection groups showed significant reduction in the chromosomal aberration frequencies (t=5.34-25.48, P<0.05), the micronucleus cell frequencies (t=5.18-29.44, P<0.05), the micronucleus frequencies (t=4.67-12.04, P<0.05), the tailleghth (t=16.18-19.64, P<0.05), the tailmoment (t=11.79-13.01, P<0.05), the olivetailmoment (t=12.44-14.88, P<0.05)and the tail DNA% of comet cells(t=12.05-17.30, P<0.05). The olivetailmoment of the two high concentrations in protective groups(1, 2 mmol/L)were significantly decreased compared with the other two KFL groups(0.2, 0.5 mmol/L), respectively (t=5.67-16.56, P<0.05). Compared with other KFL groups, the micronucleus frequencies and micronucleus cell frequencies of the 2 mmol/L KFL group was significantly decreased (t=4.23-5.57, P<0.05). Conclusions KFL may have protective effect on human peripheral blood lymphocytes in the genetic damage induced by radiation. |
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