| Wang Kai,Ma Cong,Di Chanjuan,et al.Study on the relationship between the radioprotective effect and the anti-oxidation of kojic acid[J].Chinese Journal of Radiological Medicine and Protection,2014,34(2):83-87 |
| Study on the relationship between the radioprotective effect and the anti-oxidation of kojic acid |
| Received:June 06, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.02.002 |
| KeyWords:Kojic acid Radioprotection Antioxidation DPPH |
| FundProject:国家科技重大专项资助课题（2011ZXJ0901-04C） |
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| Abstract:: |
| Objective To evaluate the relationship between the radioprotective effect and the anti-oxidation of kojic acid and provide experimental evidence for using kojic acid as a potential radioprotective medicine. Methods Male C57BL/6 mice were divided into 4 groups (normal group, irradiation group, low/high doses of kojic acid pretreated groups) by the random digits table method. Mice in normal group without any treatment, and mice in other groups were s.c. injected with a single dose of sterile distilled water, kojic acid (75 and 300 mg/kg, respectively) 27 h prior to a sublethal dose (4 Gy) of whole body gamma-irradiation. 20 mice were sacrificed at 3, 4, and 7 d post-irradiation, respectively. The activities of total superoxide dismutase (T-SOD) in serums and tissues (liver and kidney), the level of malondialdehyde (MDA), and the DNA contents and the frequency of micronuclei in femur bone marrow were assessed, and the radical-scavenging activity of kojic acid against stable DPPH· (2,2-diphenyl -1-picrylhydrazyl) was also determined. Results The activities of T-SOD in serums and tissues (liver and kidney) and the DNA content in bone marrow of mice pretreated with 300 mg/kg body weight of kojic acid were increased significantly (F= 22.63, 7.29, 67.58, 57.32, P<0.05) compared with the irradiation group, while the levels of MDA in serums and tissues (liver and kidney) and the frequency of micronuclei in bone marrow of mice pretreated with 300 mg/kg body weight of kojic acid decreased significantly (F= 26.22, 27.06, 4.51, 14.28, P<0.05) compared with the irradiation group. There was a marked radical-scavenging activity for kojic acid, and the IC50 values (which was defined as the concentration of kojic acid or BHT (2,6-di-tert-butyl-4 -methylphenol) causing 50% inhibition of absorbance) for kojic acid and BHT were (4.55±0.26)mg/ml and (1.83±0.10)mg/ml, respectively, and the results of DPPH· inhibition for kojic acid (20 mg/ml) and BHT (20 mg/ml) were (82.38±3.51)% and (96.41±0.45)%, respectively. Conclusions Kojic acid protects the hereditary substances of mice from radiation damage by scavenging free radicals in mice and exerts marked radioprotective effect. |
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