| Liu Jingjia,Wang Hao,Qu Ang,et al.Enhancement of cetuximab on radiosensitivity of colorectal cancer cells exposed to 125I seeds[J].Chinese Journal of Radiological Medicine and Protection,2014,34(1):26-29,33 |
| Enhancement of cetuximab on radiosensitivity of colorectal cancer cells exposed to 125I seeds |
| Received:March 29, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.01.007 |
| KeyWords:C225 Continuous low dose rate radiation Radiosensitization Colorectal cancer cell Cell apoptosis |
| FundProject:国家自然科学基金（81071834） |
| Author Name | Affiliation | E-mail | | Liu Jingjia | Cancer Center, Peking University Third Hospital, Beijing 100191, China | | | Wang Hao | Cancer Center, Peking University Third Hospital, Beijing 100191, China | | | Qu Ang | Cancer Center, Peking University Third Hospital, Beijing 100191, China | | | Li Jinna | Cancer Center, Peking University Third Hospital, Beijing 100191, China | | | Zhao Yong | 中国科学院动物研究所 生物膜与膜生物工程国家重点实验室 | | | Wang Junjie* | Cancer Center, Peking University Third Hospital, Beijing 100191, China | junjiew920@sohu.com |
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| Abstract:: |
| Objective To investigate the effect of cetuximab (C225) on the radiosensitivity of colorectal cancer cells CL187 and underlying mechanism. Methods Cell survival was detected by colony forming assay. The levels of apoptosis and cell cycle distribution were determined by flow cytometer. The mitotic ratio was measured by Wright's-Giemsa mixed coloring method. The protein levels of Bax and Bcl-2 were detected by Western blot. Results The sensitizing enhancement ratio of C225 was approximately 1.4. C225 treatment and 125I seed radiation induced G1 cell cycle arrest individually. C225 increased the radiation-induced apoptosis (t=6.6,P<0.05) and cellular Bax/Bcl-2 ratio (t=9.4, P<0.05), but did not increase radiation-induced G1 arrest. In addition, there was no difference in mitotic index among different groups. Conclusions C225 sensitizes CL187 to 125I seed irradiation, which might be related with increase of radiation-induced apoptosis. |
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