| Tan Wei,Yin Jiaojiao,Zhou Lijun,et al.60Co γ-ray irradiation induced expression of salt-inducible kinase 2[J].Chinese Journal of Radiological Medicine and Protection,2014,34(1):22-25 |
| 60Co γ-ray irradiation induced expression of salt-inducible kinase 2 |
| Received:March 08, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.01.006 |
| KeyWords:Salt-inducible kinase 2 Ionizing radiation Gene expression G2/M arrest |
| FundProject:国家自然科学基金（31270894，81172119） |
| Author Name | Affiliation | E-mail | | Tan Wei | The Institute for Environmental Medicine and Radiation Hygiene, College of Public Health, University of South China, Hengyang 421001, China | | | Yin Jiaojiao | 军事医学科学院放射与辐射医学研究所 | | | Zhou Lijun | 中国人民解放军海军总医院中心实验科 | | | Huang Bo | The Institute for Environmental Medicine and Radiation Hygiene, College of Public Health, University of South China, Hengyang 421001, China | | | Liu Xiaodan | 军事医学科学院放射与辐射医学研究所 | | | Wang Yu | 军事医学科学院放射与辐射医学研究所 | | | Gu Yongqing | 军事医学科学院放射与辐射医学研究所 | | | Rang Weiqing | The Institute for Environmental Medicine and Radiation Hygiene, College of Public Health, University of South China, Hengyang 421001, China | | | Zhou Pingkun* | 军事医学科学院放射与辐射医学研究所 | zhoupk@bmi.ac.cn |
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| Abstract:: |
| Objective To explore the expression changes of SIK2 in both protein and mRNA levels in response to ionizing radiation and its potential relationship with the cell cycle distribution alteration. Methods HepG2 cells were irradiated with 60Co γ-rays. Western blot and Real-time PCR were used to detect the level of the protein and mRNA, respectively. TdR double-blocking method was used to synchronize cell and the cell cycle distribution was analyzed by flow cytometry. Results The protein level of SIK2 was significantly increased at 4, 6, and 10 h (t=3.00, 3.98, 4.17, P<0.05) after 2 Gy irradiation. The same increasing tendency of SIK2 protein level was observed in the cells irradiated by 10 Gy of γ-rays, but the increased extent was less than that induced by 2 Gy. A significantly increased mRNA expression of SIK2 was detected at about 10 h after 2 or 10 Gy irradiation (t=4.54, 2.74, P<0.05). The G2/M arrest approached to a peak value at 10 h after 2 and 10 Gy irradiation. The result of mRNA level analysis indicated that there was no obvious change among different cell cycle phases of the synchronized cells. Conclusions The level of SIK2 protein can be significantly induced by both 2 Gy and 10 Gy irradiation, and a part of the increased protein level is attributed to the induction of mRNA transcription 10 h post-irradiation. The increased SIK2 mRNA expression is not the consequence of the cell cycle alteration caused by irradiation-induced G2/M arrest. |
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