| Shi Tao,Dong Juancong,Zhang Wanze,et al.Influence of ionizing radiation on ICOS/ICOSL and related signal pathway in mice spleen[J].Chinese Journal of Radiological Medicine and Protection,2014,34(1):18-21 |
| Influence of ionizing radiation on ICOS/ICOSL and related signal pathway in mice spleen |
| Received:April 15, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.01.005 |
| KeyWords:Ionizing irradiation ICOS/ICOSL NF-κB IL-10 Signaling pathway |
| FundProject:国家自然科学基金（81201737，30870584） |
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| Abstract:: |
| Objective To observe the changes of inducible co-stimulator and ligand(ICOS/ICOSL), nuclear factor NF-κB, and cytokine IL-10 in mouse spleen after different doses of ionizing radiation. Methods The healthy ICR mice were randomly divided into low dose irradiation groups with 0.05, 0.075 and 0.2 Gy and high dose irradiation groups with 1, 2, 4 and 6 Gy, respectively. The control mice were killed 16 h after sham-irradiation and the irradiated mice were killed at 0, 4, 8, 16, 24, 48, 72 h after irradiation. Mice spleen was taken out to prepare for single cell suspension and to extract total protein. The expression of ICOS/ICOSL was detected using flow cytometry. The expressions of NF-κB and IL-10 were detected by Western blot and ELISA, respectively. Results After low doses of 0.05 and 0.075 Gy, the percentage of ICOS/ICOSL double positive cells was significantly lower than that of sham-irradiation (t=4.743, 4.120,P<0.01). The expression of ICOS/ICOSL was increased after high doses of 4 and 6 Gy irradiation (t=-4.950, -7.310,P<0.01). The NF-κB expression had a similar tendency with ICOS/ICOSL. But the secretion of IL-10 was decreased after low dose of 0.075 and 0.2 Gy irradiation (t=5.277, 2.854,P<0.05),but increased after 6 Gy irradiation compared with sham-irradiation group (t=7.196, P<0.01). Conclusions Low dose irradiation inhibits the expressions of ICOS/ICOSL, NF-κB and IL-10,while high dose irradiation inscreases the expression of ICOS/ICOSL and activates NF-κB then increases the secretion of IL-10. |
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