| Guo Yanting,Wang Yan,Du Liqing,et al.The radiosensitization effect of Tat-SmacN7 on breast cancer cell lines[J].Chinese Journal of Radiological Medicine and Protection,2014,34(1):13-17 |
| The radiosensitization effect of Tat-SmacN7 on breast cancer cell lines |
| Received:March 29, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.01.004 |
| KeyWords:Tat-SmacN7 Breast cancer Radiosensitization Cell apoptosis Inhibitor of apoptosis proteins |
| FundProject:国家自然科学基金（31240052）；天津市自然科学基金（13JCYBJC23500，13JCQNJC11600）；卫生行业科研专项（201002009） |
| Author Name | Affiliation | E-mail | | Guo Yanting | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Wang Yan | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Du Liqing | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Xu Chang | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Cao Jia | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Liu Jianxiang | 中国CDC辐射防护与核安全医学所 | | | Chen Fenghua | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Fu Yue | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | Liu Qiang* | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | liuqiang@irm-cams.ac.cn | | Su Xu | 中国CDC辐射防护与核安全医学所 | |
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| Abstract:: |
| Objective To investigate the radiosensitive effect of Tat-SmacN7 on human breast cancer cell line of SK-BR-3 and the underlying mechanism. Methods SK-BR-3 cells in the logarithmic phase were divided into 4 groups: control group, drug group, 137Cs γ-ray radiation group and the case group (γ-ray radiation+Tat-SmacN7). MTT assay was performed to evaluate the cytotoxicity of Tat-SmacN7. Colony formation assay was used to measure cell survival fraction. A single-hit multi-target model was used to fit the survival curve and calculate the sensitive enhancement ratio (SER). Apoptosis rate was analyzed by using flow cytometry. The expressions of inhibitor of apoptosis proteins(IAP) including XIAP, cIAP-1 proteins were detected by Western blot method. Results Tat-SmacN7 inhibited cell growth in a dose-dependent manner(r=0.924,P<0.05)and its 50% inhibition concentration(IC50)was(62.62±1.19)μmol/L,and IC20 was(5.66±0.67)μmol/L. In addition, 5 μmol/L Tat-SmacN7 could enhance cell radiosensitivity with a SER of 1.48. After 6 Gy γ-ray radiation at 48 h, the incidence of apoptosis in the case group was higher than that in the irradiation alone group (t=7.01,P<0.05). Tat-SmacN7 only inhibited XIAP expression, whereas Tat-SmacN7 combined with radiation inhibited cIAP-1 as well. Conclusions Tat-SmacN7 has a radiosensitization effect on human breast cancer SK-BR-3 cell by promoting apoptosis, which might be related with XIAP expression. |
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