| Liu Jing,Tang Yiting,Zhou Jundong,Zhang Shuyu,Cao Han,Wu Jinchang,Luo Judong,Chen Guanglie,Cao Jianping*.Inhibition effect of proteasome inhibitor MG132 combined with X-ray irradiation on cell growth, metastasis and cycle distribution of human lung adenocarcinoma cells[J].Chinese Journal of Radiological Medicine and Protection,2014,34(1):8-12 |
| Inhibition effect of proteasome inhibitor MG132 combined with X-ray irradiation on cell growth, metastasis and cycle distribution of human lung adenocarcinoma cells |
| Received:April 26, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.01.003 |
| KeyWords:Proteasome inhibitor MG132 Lung adenocarcinoma Cell growth Metastasis Cell cycle arrest |
| FundProject:国家自然科学基金（81372433，81172597，81102078）；江苏省高校重大科研项目（11KJA310001） |
| Author Name | Affiliation | E-mail | | Liu Jing | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | Tang Yiting | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | Zhou Jundong | 南京医科大学附属苏州医院放疗科 | | | Zhang Shuyu | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | Cao Han | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | Wu Jinchang | 南京医科大学附属苏州医院放疗科 | | | Luo Judong | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | Chen Guanglie | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | Cao Jianping* | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | jpcao@suda.edu.cn |
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| Abstract:: |
| Objective To study the effects of proteasome inhibitor MG132 on the growth, metastasis, and cell cycle distribution of human lung adenocarcinoma cells A549 irradiated by X-rays. Methods After treatment of MG132 and irradiation, cell proliferation was detected by MTT assay. Survival was measured by clonogenic assay. Cell migration ability was detected by the Scratch migration assay. Cell invasion ability was detected by transwell migration assay. Cell cycle distribution were analyzed by flow cytometry assay. Protein expression was detected by Western blot assay. Results MG132 alone inhibited cell growth in a dose-and time-dependent manner. MG132 in combination with radiation significantly suppressed the growth, migration and invasion of A549 cells compared to the control(F=554.78, 954.64,P<0.01). MG132 enhanced radiation-induced G1-arrest(t=4.44, 12.41, 3.52, 6.72, P<0.05). The G1 cell cycle distribution rate of MG132 plus RT group was increased to (71.05±4.17)%. The expressions of MMP-2, MMP-9 and Cyclin D1 were significantly suppressed by MG132 in combination with radiation, while the expression of P53 was up-regulated. Conclusions MG132 inhibits cell growth, migration and invasion ability, and induces G1 cell cycle arrest of A549 cells treated with MG132 in combination with radiation, in which the down-regulation of MMPs and Cyclin D1 and up-regulation of P53 may be involved. |
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