| JIA Yong-shi,LIN Bai-hua,BI Ai-hong,et al.Prevention effect of carnosine on radiation-induced lung injury in mice[J].Chinese Journal of Radiological Medicine and Protection,2013,33(6):607-610 |
| Prevention effect of carnosine on radiation-induced lung injury in mice |
| Received:April 14, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.06.010 |
| KeyWords:Carnosine Lung injury Prevention TGF-β1 |
| FundProject:浙江省医药卫生科技计划项目（2010KYA017） |
| Author Name | Affiliation | | JIA Yong-shi | Department of Radiation Oncology, Zhejiang Province People's Hospital, Hangzhou 310014, China | | LIN Bai-hua | Department of Radiation Oncology, Zhejiang Province People's Hospital, Hangzhou 310014, China | | BI Ai-hong | Department of Radiation Oncology, Zhejiang Province People's Hospital, Hangzhou 310014, China | | ZHAN Wen-ming | Department of Radiation Oncology, Zhejiang Province People's Hospital, Hangzhou 310014, China | | ZHANG Li-ping | Department of Radiation Oncology, Zhejiang Province People's Hospital, Hangzhou 310014, China | | XU Li-ping | Department of Radiation Oncology, Zhejiang Province People's Hospital, Hangzhou 310014, China | | LI Qiang | Department of Radiation Oncology, Zhejiang Province People's Hospital, Hangzhou 310014, China |
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| Abstract:: |
| Objective To investigate the protective effect of carnosine on radiation-induced lung injury in mice. Methods A total of 108 C57/BL female mice were randomly divided into 4 groups:control group without treatment, irradiation alone group, irradiation + carnosine group (15 mg·kg-1·d-1), and carnosine alone group (15 mg·kg-1·d-1). There were 18 mice in control group and 30 mice in every other group.Whole lung anterior chest was irradiated with a single dose of 13 Gy 10 MV X-rays. The mice were administered with carnosine (15 mg·kg-1·d-1) at 30 minutes before irradiation and then gavaged once a day until the end of the experiment.The control group was given with saline. At 7, 28, and 56 d after irradiation, 6 mice of control group and 10 mice of each other group were killed. A portion of lung tissues were stained with HE and other part of lung tissues were used to detect the levels of SOD. Meanwhile, TGF-β1 and TNF-α in the serum were detected with ELISA. Results Different levels of inflammation factors were expressed in the lung tissues of irradiation group and irradiation + carnosine group at 56 d after irradiation, but the inflammation in the irradiation + carnosine group was significantly lighter than that in the irradiation group. Among (7, 28 and 56 d) after radiation, TGF-β1, TNF-α, and SOD levels in different groups had significant differences. At the same time point after irradiation, the level of SOD in lung tissue of irradiation + carnosine group was significantly higher than that of irradiation group(F=4.33,4.19,3.34,P<0.05), but the levels of TGF-β1 and TNF-α in serum were reduced. ConclusionsCarnosine can prevent and inhibit radiation-induced lung injury in mice by increasing SOD and reducing TGF-β1 and TNF-α. |
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