| ZUO Ya-hui,DANG Xu-hong,ZHANG Hui-fang,et al.Study on serum proteomics in rats after accumulated irradiation with 137Cs γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2013,33(6):597-601 |
| Study on serum proteomics in rats after accumulated irradiation with 137Cs γ-rays |
| Received:March 21, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.06.008 |
| KeyWords:Accumulated irradiation Serum proteomics Isobaric tags for relative and absolute quantitation (iTRAQ) Interaction network |
| FundProject: |
| Author Name | Affiliation | | ZUO Ya-hui | China Institute for Radiation Protection, Taiyuan 030006, China | | DANG Xu-hong | China Institute for Radiation Protection, Taiyuan 030006, China | | ZHANG Hui-fang | China Institute for Radiation Protection, Taiyuan 030006, China | | LIU Jian-gong | China Institute for Radiation Protection, Taiyuan 030006, China | | YANG Biao | China Institute for Radiation Protection, Taiyuan 030006, China | | WANG Chao | China Institute for Radiation Protection, Taiyuan 030006, China | | DUAN Zhi-kai | China Institute for Radiation Protection, Taiyuan 030006, China |
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| Abstract:: |
| Objective To investigate the changes of proteomics in serum of Sprague-Dawley(SD)rats after accumulated irradiation with 137Cs γ-rays. Methods A total of thirty mature SD rats were randomly divided into three groups: 0.2 Gy group, 2 Gy group and healthy control group. Rats were irradiated at a dose rate of 0.336 mGy/min for 10 d and 20 d continuously. Isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the different protein expression in serum of irradiated rats. Gene Ontology, KEGG pathway and protein-protein interaction network analysis were conducted using softwares. Results In total, 363 protein spots were identified. Twenty nine proteins were differentially expressed in both groups compared with control, of which 10 proteins were up-regulated and 19 proteins were down-regulated. Based on the information of GO categories,these differentially expressed proteins were mainly located in the cytoplasm and membrane concerning the function of binding and catalytic activity. Analysis with the PAJEK software demonstrated that 16 differentially expressed proteins could form a complicated interaction network where glutathione S-transferase P1 (GSTP1), phosphoglycerate kinase 1(PGK1) and protein disulfide-isomerase (PDI) might be key nodes.Conclusions Accumulated irradiation can induce differentially expressed proteins in serum of irradiated rats. Analysis on functional roles of the screened proteins GSTP1, PGK1 and PDI may provide insight into further mechanistic investigations and underlying molecular biomarkers induced by accumulated irradiation. |
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