| DU Li-qing,WANG Yan,XU Chang,et al.Radiosensitization effect and underlying mechanism of ANTP-SmacN7 fusion peptide on tumor cells[J].Chinese Journal of Radiological Medicine and Protection,2013,33(6):569-573 |
| Radiosensitization effect and underlying mechanism of ANTP-SmacN7 fusion peptide on tumor cells |
| Received:February 20, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.06.001 |
| KeyWords:ANTP-SmacN7 fusion protein Apoptosis Radiosensitization Tumor cell |
| FundProject:国家自然科学基金（81302803，31240052）；天津市自然科学基金（13JCYBJC23500）；教育部博士点基金（20121106120043）；中央高校基本科研业务费专项资金（2012G01） |
| Author Name | Affiliation | E-mail | | DU Li-qing | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | WANG Yan | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | XU Chang | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | CAO Jia | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | FU Yue | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | CHEN Feng-hua | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | GUO Yan-ting | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | LIU Qiang | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | qiangliuohn@163.com | | FAN Fei-yue | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | FAN Sai-jun | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | |
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| Abstract:: |
| Objective To explore the sensibilization effect and underlying mechanism of ANTP-SmacN7 fusion peptide on tumor cell radiation. Methods ANTP-SmacN7 fusion protein was synthesized and its ability of cell permeation was detected. Colony formation assay was used to detect the survival fraction of tumor cells exposed to ANTP-SmacN7. Annexin V-FITC/PI assay was used to detect the cell apoptosis after radiation and ANTP-SmacN7 exposure. Western blot assay was used to detect the expressions of Caspase-8, Caspase-9 and Caspase-3 before and after ANTP-SmacN7 exposure as well as the expression of XIAP in the irradiated cells. Results There was a negative relationship between XIAP expression and radiosensitivity of tumor cells(r=0.82). ANTP-SmacN7 could be accumulated in tumor cells and enhanced cell radiosensitivity with a SER of 1.41. ANTP-SmacN7 could also accelerate the apoptosis in the cells highly expressing XIAP after irradiation with 4, 8 and 10 Gy (t=-14.924,-7.294 and -15.866, P<0.05). In addition, ANTP-SmacN7 could activate Caspase-3 but did not influence radiation-induced expression of Caspase-3 in the cells. Conclusion ANTP-SmacN7 fusion protein can weaken radioresistance of tumor cells by activating Caspase-3. |
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