| YANG Bai-xia,YANG Xi,ZHU Qi-wei,WU Zhi-jun,ZHAI Xiao-gang,XIA Xiao-chun,CAI Jing.Radiosensitivity of berberine on hypoxia esophageal cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2013,33(5):484-488 |
| Radiosensitivity of berberine on hypoxia esophageal cancer cells |
| Received:January 31, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.007 |
| KeyWords:Berberine Esophageal cancer Hypoxia HIF-1 Radiosensitivity |
| FundProject: |
| Author Name | Affiliation | E-mail | | YANG Bai-xia | Nantong Tumor Hospital, Nantong 226361, China | | | YANG Xi | Nantong Tumor Hospital, Nantong 226361, China | | | ZHU Qi-wei | Nantong Tumor Hospital, Nantong 226361, China | | | WU Zhi-jun | Nantong Tumor Hospital, Nantong 226361, China | | | ZHAI Xiao-gang | Nantong Tumor Hospital, Nantong 226361, China | | | XIA Xiao-chun | Nantong Tumor Hospital, Nantong 226361, China | | | CAI Jing | Nantong Tumor Hospital, Nantong 226361, China | cj7227@sina.com |
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| Abstract:: |
| Objective To explore the radiosensitivity of berberine on esophageal cancer cells under hypoxia condition.Methods MTT assay and clonogenic survival assay were used to evaluate the effect of berberine on proliferation inhibition and radiosensitivity of esophageal cancer cells, respectively. Immunofluorescence was employed to examine the expression of HIF-1. The change of cell cycle distribution and cell apoptosis was assayed by flow cytometry. The expression of HIF-1 was measured by Western blot. DNA damage was detected by γ-H2AX Foci counting.Results With a clear dose and time effect, berberine inhibited cell proliferation and enhanced cell radiosensitivity (t=3.69, P<0.05) with a sensitizing enhancement ratio (SER) of 1.42. Berberine caused a dose-dependent decrease in HIF-1 protein expression and also significantly increased the cell apoptosis in ECA-109 population (t=4.74,P<0.05). Compared with the radiation alone group, berberine enhanced X-ray induced DNA double chain breaks (DSB). Conclusions Berberine can increase the radiosensitivity of esophageal cell line ECA-109, which may be associated with decrease of HIF-1 expression and induction of apoptosis in ECA-109 cells. |
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