| CHI Cui-ping,TIAN Rong,LIU Hui-fang,WANG Hai-yan,WEI Jin-ping,GUO Jian-ping,GUO Feng-ling,LI Shu-fang.Follow-up study of biological indicators in three accidently-exposed patients[J].Chinese Journal of Radiological Medicine and Protection,2013,33(5):480-483 |
| Follow-up study of biological indicators in three accidently-exposed patients |
| Received:March 21, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.006 |
| KeyWords:Accidental exposure Gene expression profile CD4+/CD8+ ratio Lymphocyte subpopulations Albumin |
| FundProject: |
| Author Name | Affiliation | | CHI Cui-ping | China Institute for Radiation Protection, Taiyuan 030006, China | | TIAN Rong | China Institute for Radiation Protection, Taiyuan 030006, China | | LIU Hui-fang | China Institute for Radiation Protection, Taiyuan 030006, China | | WANG Hai-yan | China Institute for Radiation Protection, Taiyuan 030006, China | | WEI Jin-ping | China Institute for Radiation Protection, Taiyuan 030006, China | | GUO Jian-ping | China Institute for Radiation Protection, Taiyuan 030006, China | | GUO Feng-ling | China Institute for Radiation Protection, Taiyuan 030006, China | | LI Shu-fang | China Institute for Radiation Protection, Taiyuan 030006, China |
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| Abstract:: |
| Objective To identify the candidate biomarkers for early diagnosis or the therapeutic targets in late ionizing radiation injury by analyzing the lymphocyte subgroups, serum protein levels and gene expression profiles in the peripheral blood of three60Co γ-ray accidentally-exposed patients during three years after irradiation. Methods Flow cytometry and agarose gel electrophoresis were used to investigate lymphocyte subgroups and serum protein levels, respectively. Gene expression profiles were obtained by using whole genome gene expression chip assay. Results Three-years after irradiation, the percentage of CD4+ T lymphocytes declined and the percentage of CD8+ T lymphocytes increased so that the ratio of CD4+ to CD8+ decreased, meanwhile, the serum albumin level declined whereas beta globulin increased in the patient peripheral blood. In addition, the expressions of 285 genes were up-regulated while those of 446 genes were down-regulated in the irradiated patient samples, where the expressions of KDR, CEACAM8 and OSM were further confirmed by real-time RT-PCR. Conclusions The changes of the percentage of CD4+ T cells and the ratio of CD4+/CD8+ T cells in peripheral blood may be useful as biomarkers of late radiation injury. |
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