| LI Xiao,JIANG Qi-sheng,LI Feng-sheng,LÜ Jin,WANG Si-nian,HE Rui,ZOU Yue,SUN Song-wei,WANG Cheng,LIU Jia-zhao.Effect of low dose X-ray irradiation on migration of human dendrite cells and underlying mechanism[J].Chinese Journal of Radiological Medicine and Protection,2013,33(5):476-479 |
| Effect of low dose X-ray irradiation on migration of human dendrite cells and underlying mechanism |
| Received:January 18, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.005 |
| KeyWords:Irradiation Dendritic cells Migration CCR7 |
| FundProject:国家自然科学基金(81172130/H1610);国家自然科学基金青年项目(81202151) |
| Author Name | Affiliation | E-mail | | LI Xiao | The Second Artillery General Hospital, Beijing 100088, China | | | JIANG Qi-sheng | The Second Artillery General Hospital, Beijing 100088, China | jqs598@sina.com | | LI Feng-sheng | The Second Artillery General Hospital, Beijing 100088, China | | | LÜ Jin | The Second Artillery General Hospital, Beijing 100088, China | | | WANG Si-nian | The Second Artillery General Hospital, Beijing 100088, China | | | HE Rui | The Second Artillery General Hospital, Beijing 100088, China | | | ZOU Yue | The Second Artillery General Hospital, Beijing 100088, China | | | SUN Song-wei | The Second Artillery General Hospital, Beijing 100088, China | | | WANG Cheng | The Second Artillery General Hospital, Beijing 100088, China | | | LIU Jia-zhao | The Second Artillery General Hospital, Beijing 100088, China | |
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| Abstract:: |
| Objective To study the effect of low dose X-ray irradiation on the migration of human dendritic cells and its mechanism in vitro. Methods The human peripheral blood mononuclear cells (PBMC) were separated and treated with rhGM-CSF and rhIL-4 in order to differentiate them to dendritic cells (DC) in vitro. To enhance cell maturation, 50 mg/L TNF-α was added into the medium at 5 d of culture. At 6 d of cell culture, DCs were radiated with X-rays of 0.05, 0.1, 0.2 and 0.5 Gy, respectively. At 8 d, the dendrite cells were collected for further analysis. The expressions of CC-Chemokine Receptor 7 (CCR7) mRNA and its protein were detected by RT-PCR and Western blot, respectively. Transwell culture inserts were used to measure the amount of migrated cells. Results After 0.2 and 0.5 Gy radiation, the expression of CCR7 mRNA of DCs was remarkably increased(t=14.72, 4.72,P<0.05), but the expression of CCR7 protein in the DCs irradiated with 0.2 Gy was higher than that irradiated with other doses(t=4.46, P<0.05), meanwhile the amount of migrated DCs was obviously increased(t=2.95, P<0.05). Furthermore, DCs treated with anti-CCR7 monoclone antibody decreased the ability of radiation-induced migration(t=4.63-8.96,P<0.05). Conclusions 0.2 Gy X-ray irradiation significantly can enhance the migration ability of DCs in vitro, which may be correlated with the increase of CCR7 expression. |
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