| LI Feng-sheng,GAO Ling,SONG Xiu-jun,LI Xiao,JIANG Qi-sheng,CHEN Xiao-hua.Effect of ionizing radiation on the expressions of STAT3 target genes in A549 cells[J].Chinese Journal of Radiological Medicine and Protection,2013,33(5):472-475 |
| Effect of ionizing radiation on the expressions of STAT3 target genes in A549 cells |
| Received:May 16, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.004 |
| KeyWords:Ionizing radiation STAT3 VEGF Survivin |
| FundProject:国家自然科学基金(8120215,81172130,81001216) |
| Author Name | Affiliation | E-mail | | LI Feng-sheng | The PLA Research Center for Medical Monitoring and Prevention of Nuclear Radiation Damage, The Second Artillery General Hospital, Beijing 100088, China | | | GAO Ling | 中国疾病预防控制中心辐射防护与核医学安全所 辐射防护与核应急中国疾病预防控制中心重点实验室 | | | SONG Xiu-jun | The PLA Research Center for Medical Monitoring and Prevention of Nuclear Radiation Damage, The Second Artillery General Hospital, Beijing 100088, China | | | LI Xiao | The PLA Research Center for Medical Monitoring and Prevention of Nuclear Radiation Damage, The Second Artillery General Hospital, Beijing 100088, China | | | JIANG Qi-sheng | The PLA Research Center for Medical Monitoring and Prevention of Nuclear Radiation Damage, The Second Artillery General Hospital, Beijing 100088, China | | | CHEN Xiao-hua | 军事医学科学院放射与辐射医学研究所 | Chenxh0676@sina.com |
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| Abstract:: |
| Objective To investigate the effect of ionizing radiation on the expression of transcription factor STAT3 target genes in the pulmonary adenocarcinoma cell line A549. Methods The expressions of VEGF, Bcl-2 and Survivin in A549 cells with or without STAT3 inhibition were detected by RT-PCR and/or Western blot 24 h after 2 or 4 Gy of γ-ray irradiation. Results After γ-ray irradiation with 2 or 4 Gy, the expression of VEGF and Survivin increased significantly. However, the expression of Bcl-2 was not affected by γ-ray irradiation. Furthermore, the increased expression of VEGF and Survivin induced by radiation was found to be inhibited after radiation-induced activation of STAT3 was blocked by AG490 inhibiter. Conclusions γ-ray irradiation could up-regulate the expression of Survivin and VEGF via activating STAT3. The increase of Survivin might partly inhibit the radiation-induced apoptosis of A549 cells, and the up-regulation of VEGF might contribute to the tumor angiogenesis. |
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