| ZHOU Hui,YANG Bing,TANG Quan,SUN Yuan-ming,HAN Ying,FAN Fei-yue,JIA Li-li.Effect of ionizing radiation on the expressions of RANKL and OPG mRNA and proteins in osteoblasts[J].Chinese Journal of Radiological Medicine and Protection,2013,33(5):468-471 |
| Effect of ionizing radiation on the expressions of RANKL and OPG mRNA and proteins in osteoblasts |
| Received:April 08, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.003 |
| KeyWords:Ionizing radiation Osteoblasts Osteoclasts Receptor activator for NF-κB ligand Osteoprotegerin |
| FundProject:国家自然科学基金(30970867) |
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| Abstract:: |
| Objective To investigate the effect of radiation on the expressions of RANKL and OPG in osteoblasts in order to disclose the molecular mechanism of bone injury induced by ionizing radiation. Methods The osteoblasts were differentiated from MC3T3-E1 cells. After 2 or 4 Gy137Cs γ-irradiation, the mRNA and protein expression levels of RANKL and OPG of osteoblast precursor and osteoblast were detected by real-time PCR and Western blot. Results The expressions of RANKL mRNA (t=5.41, P<0.05) and protein (t=68.37, P<0.01) were up-regulated after 4 Gy irradiation, while the expressions of OPG mRNA (t=5.20,7.02,P<0.05) and protein (t=7.78,9.45,P<0.05) were down-regulated after 2 and 4 Gy irradiation. Conclusions 2 and 4 Gy ionizing radiation alters RANKL/RANK/OPG pathway in osteoblasts, which may promote the osteoclast differentiation and maturation and hence promote bone resorption of osteoclasts. |
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