| GU Yuan,TANG Xin-xing,ZHANG Yuan-yuan,YAO Xue-ting,WANG Chang.Alternations of phospholipids in the rat intestinal epithelial cells after ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2013,33(5):457-462 |
| Alternations of phospholipids in the rat intestinal epithelial cells after ionizing radiation |
| Received:January 04, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.001 |
| KeyWords:Ionizing radiation Intestinal epithelial cells Phospholipid |
| FundProject:国家自然科学基金(31000385);江苏省高校自然科学研究项目(10KJB310012);江苏省博士后基金(0901052C);大学生创新创业训练计划(2012yd016) |
| Author Name | Affiliation | E-mail | | GU Yuan | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | TANG Xin-xing | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | ZHANG Yuan-yuan | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | YAO Xue-ting | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | WANG Chang | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | wangchang@suda.edu.cn |
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| Abstract:: |
| Objective To investigate radiation-induced alternations of phospholipids in epithelial cells, and to provide experimental evidence for understanding the mechanism of radiation-induced intestinal injury. Methods The intestinal epithelial cells (IEC-6) in rats were divided into three groups: normal control group, 8 Gy X-ray irradiation group and 12 Gy X-ray irradiation group. Phospholipids were extracted at 6 h or 24 h after radiation and then measured by high-performance liquid chromatography and mass spectrometry (HPLC-MS). Results At 6 h after radiation, the phospholipids in 8 Gy irradiation group didn't vary significantly, while those in 12 Gy irradiation group changed. The PG, PI and Lyso PC were significantly up-regulated (F=5.37, 9.60, 9.88, P<0.05). However, at 24 h after radiation, many PE and PG species in both irradiation groups declined (F=5.15-99.77, P<0.05) and SM species increased in 12 Gy irradiation group (F=4.35-7.92, P<0.05). Conclusions The ionizing radiation could disorder phospholipid metabolism in IEC-6 cells with a dose-dependent manner. |
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