| MA Hui,DING Jian-hua,ZHANG Sheng-bin,et al.Evaluation the regeneration effect of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis[J].Chinese Journal of Radiological Medicine and Protection,2013,33(4):373-375,387 |
| Evaluation the regeneration effect of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis |
| Received:January 28, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.04.009 |
| KeyWords:Radiation enteritis Liang-Xue-Yu-Chang decoction Proliferating cell nuclear antigen (PCNA) Ki67 Bromodexyrldine (BrdU) |
| FundProject:中国人民解放军全军医学科研"十二五"基金(cws11J032) |
| Author Name | Affiliation | E-mail | | MA Hui | Department of Colorectal Surgery, Licentiate Training Base for Liaoning Medical College, General Hospital of the Second Artillery Corps of Chinese PLA, Beijing 100088, China | | | DING Jian-hua | Department of Colorectal Surgery, Licentiate Training Base for Liaoning Medical College, General Hospital of the Second Artillery Corps of Chinese PLA, Beijing 100088, China | | | ZHANG Sheng-bin | Department of Colorectal Surgery, Licentiate Training Base for Liaoning Medical College, General Hospital of the Second Artillery Corps of Chinese PLA, Beijing 100088, China | | | ZHAO Ke | 内蒙古包钢医院普外科 | shanmu1023@yahoo.com.cn |
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| Abstract:: |
| Objective To study the effects of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis. Methods Sixty male SD rats were divided into three groups randomly: normal rats as control, abdominal radiation control group given distilled water after 10 Gy radiation, and abdominal radiation plus drug group given Liang-Xue-Yu-Chang decoction after 10 Gy radiation. Drugs or distilled water were chronically administered to animals for 7 days. After that, 5-bromodexyridine (BrdU) was injected into abdominal cavity, SP immunohistochemistry was used to evaluate intestinal epithelium proliferating cell nuclear antigen (PCNA) and the positive cells labeled with Ki67 and BrdU. Results The number of intestinal epithelium cells with PCNA expression, positive Ki67 and BrdU were 22.0±2.7, 71.2±5.7, and 26.2±5.9 respectively in the drug treatment group, significantly higher than those in the abdominal radiation control group (11.2±1.9, 61.6±5.5, and 11.3±2.2)(t=14.629, 5.420, 11.582, P<0.05),but lower than those in the normal control group(30.4±5.7, 86.6±5.1, and 32.3±3.2)(t=14.291, 9.004, 4.731, P<0.05). Conclusion Liang-Xue-Yu-Chang decoction could improve the proliferation of intestinal epithelium in the rats with radiation enteritis. |
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