| LIU Lan-tao,LIU Jian-xiang,SU Xu.Mechanism of 2 Gy 60Co γ-ray-induced apoptosis in the regulatory T cells of mice[J].Chinese Journal of Radiological Medicine and Protection,2013,33(4):364-367 |
| Mechanism of 2 Gy 60Co γ-ray-induced apoptosis in the regulatory T cells of mice |
| Received:January 25, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.04.007 |
| KeyWords:Ionizing radiation Regulatory T cell Apoptosis CD39 |
| FundProject:卫生行业科研专项(201002009) |
| Author Name | Affiliation | E-mail | | LIU Lan-tao | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Jian-xiang | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | SU Xu | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To analyze the influence of ionizing radiation on the viability of the regulatory T cells and the relationship between the expression of CD39 and apoptosis related factors in mice Treg cells. Methods Mice were administered to whole body irradiation with 2 Gy of γ-rays, then lymphocytes were separated from thymus and spleen. The percentages of CD4+ T and CD4+ FOXP3+ CD25+ Treg lymphocytes, apoptosis related genes of Bax,Bcl-2 and CD39 were analyzed with FACS. Results Treg cells had a higher survival fraction than CD4+FOXP3-Tcon cells after irradiation, and the time-responses of Treg cells in thymus and spleen were different. The apoptosis rate of Treg cells increased at 4 d after irradiation(t=-3.6,-7.4, P<0.05). The Bax/Bcl-2 ratio and the CD39 expression were increased in thymus Treg cells (t=-3.4,-6.6, P<0.05), but decreased in spleen Treg cells (t=2.4, 2.6, P<0.05). Conclusions The changes of apoptosis and Bax/Bcl-2 are in consistent with the expression of CD39 in the irradiated Treg cells. |
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