| BAI Ming-hua,ZHANG Yang,WANG Bao-feng,MA Hong-bing.Mechanism of curcumin-enhanced radiosensitivity in pancreatic cancer cells PANC-1[J].Chinese Journal of Radiological Medicine and Protection,2013,33(4):360-363 |
| Mechanism of curcumin-enhanced radiosensitivity in pancreatic cancer cells PANC-1 |
| Received:January 11, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.04.006 |
| KeyWords:Curcumin γ-rays PANC-1 cells P21 Pancreatic cancer |
| FundProject:西安市科技计划医学攻关项目(GG06187) |
| Author Name | Affiliation | E-mail | | BAI Ming-hua | Department of Oncology, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China | | | ZHANG Yang | Department of Oncology, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China | | | WANG Bao-feng | Department of Oncology, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China | | | MA Hong-bing | Department of Oncology, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China | baimnh2004@126.com |
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| Abstract:: |
| Objective To investigate the combination effect of curcumin and γ-ray irradiation on PANC-1 cells in vitro. Methods PANC-1 cells were exposed to γ-rays in the presence or absence of curcumin. MTT assay was performed to evaluate cell viability. The expression of P21 was evaluated with RT-PCR and Western blot. Cell cycle distribution and apoptosis were tested by flow cytometry. Results Compared with the γ-ray irradiation group, combination treatment of curcumin and irradiation decreased the cell viability (t=6.72, P<0.01) and increased the percentage of cells in S-phase (t=4.78, P<0.05), apoptosis rate (t=6.58,P<0.01), P21 protein and mRNA expression (t=5.72, 5.63, P<0.01) in PANC-1 cells. Conclusions Curcumin increases the radiosensitivity of PANC-1 cells, which may have clinical implication on radiotherapy of pancreatic cancer. |
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