| SUN Zhi-qiang,YU Jing-ping,ZHANG Zhi-ming,WANG Jian,LI Dong-qing,MENG Qing-hong,SUN Su-ping.Effect of recombinant human endostatin on the radiosensitivity of esophageal carcinoma cells and underlying mechanism[J].Chinese Journal of Radiological Medicine and Protection,2013,33(4):346-350 |
| Effect of recombinant human endostatin on the radiosensitivity of esophageal carcinoma cells and underlying mechanism |
| Received:December 21, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.04.003 |
| KeyWords:Recombinant human endostatin Esophageal carcinoma cells Radiosensitivity |
| FundProject:江苏省卫生厅指导性科研项目(Z201220);常州市卫生局重大项目(ZD201105);常州市科技支撑社会发展项目(CE20125021) |
| Author Name | Affiliation | E-mail | | SUN Zhi-qiang | Department of Radiation Oncology, No.2 People's Hospital of Changzhou, Changzhou 213001, China | | | YU Jing-ping | Department of Radiation Oncology, No.2 People's Hospital of Changzhou, Changzhou 213001, China | | | ZHANG Zhi-ming | Department of Radiation Oncology, No.2 People's Hospital of Changzhou, Changzhou 213001, China | | | WANG Jian | Department of Radiation Oncology, No.2 People's Hospital of Changzhou, Changzhou 213001, China | | | LI Dong-qing | Department of Radiation Oncology, No.2 People's Hospital of Changzhou, Changzhou 213001, China | | | MENG Qing-hong | Department of Radiation Oncology, No.2 People's Hospital of Changzhou, Changzhou 213001, China | | | SUN Su-ping | Department of Radiation Oncology, No.2 People's Hospital of Changzhou, Changzhou 213001, China | ssp56@126.com |
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| Abstract:: |
| Objective To study the effect of recombinant human endostatin(rhES)on the radiosensitivity of esophageal squamous cells KYSE-150 and its preliminary mechanism. Methods Cells were divided into four groups: control group without treatment, rhES group treated with recombinant human endostatin, radiation alone group exposed with X-rays, and combination group exposed with X-rays plus endostatin. Colony formation assay was used to measure cell survival fraction. A single-hit multi-target model was used to fit cell survival curve and calculate the sensitive enhancement ratio (SER). Influence of rhES combined with X-ray radiation on cell cycle and apoptosis was measured by flow cytometry. Expressions of Cyclin B1, Cyclin D1, Bcl-2 and Bax mRNAs were determined by RT-PCR. Protein expressions of HIF-1α, VEGF, and VEGFR were determined by Western blot. Results D0, Dq and SF2 value of KYSE-150 cells decreased along with the concentration of rhES. At D0 dose, the SER for 100 and 200 μg/ml rhES was 1.14 and 1.27, respectively. Compared with the radiation alone group, the apoptosis rate and bax expression increased, while the expressions of VEGF and HIF-1α decreased in the combination group (t=7.97, 3.02, 117.55, 7.22, P<0.05). Conclusions rhES has radiosensitive effect on esophageal carcinoma cells KYSE-150 in vitro by inhibiting the expressions of HIF-1α and VEGF, regulating bax expression, and inducing apoptosis. |
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