| CHEN Xiao-yu,ZHU Ai-zhen,LIU Cheng-cheng,ZHU Jin-can,LIU Ge-xiu.Protective effect of 1,25-(OH)2D3 on radiation-induced bone marrow microenvironment injury[J].Chinese Journal of Radiological Medicine and Protection,2013,33(4):337-340 |
| Protective effect of 1,25-(OH)2D3 on radiation-induced bone marrow microenvironment injury |
| Received:January 29, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.04.001 |
| KeyWords:Bone marrow microenvironment Radiation Adipogenesis 1,25-(OH)2D3 |
| FundProject:国家自然科学基金(81270568) |
| Author Name | Affiliation | E-mail | | CHEN Xiao-yu | Institute of Hematology, Jinan University, Guangzhou 510632, China | | | ZHU Ai-zhen | Institute of Hematology, Jinan University, Guangzhou 510632, China | | | LIU Cheng-cheng | Institute of Hematology, Jinan University, Guangzhou 510632, China | | | ZHU Jin-can | Institute of Hematology, Jinan University, Guangzhou 510632, China | | | LIU Ge-xiu | Institute of Hematology, Jinan University, Guangzhou 510632, China | tliugx@jnu.edu.cn |
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| Abstract:: |
| Objective To investigate the protective effect of 1,25-(OH)2D3 on radiation-induced bone marrow microenvironment injury and to explore the related molecular mechanism. Methods Sixty 7-week old male BALB/c mice were randomly divided into control group without any treatment; radiation group exposed to 6.0 Gy 60Co γ-rays with DMSO, and 1,25-(OH)2D3+radiation group treated with 1,25-(OH)2D3 2.5 μg/kg dissolved in DMSO each day and 6 Gy of γ-rays. The body weight and peripheral white blood cells, femur bone marrow histology, and the proportion of adipocyte area were measured. The expression of peroxisome proliferator-activated receptor-gamma (PPARγ) was detected immunohistochemistrically at 8 d after irradiation. Results After irradiation, the number of white blood cells and the body weight decreased obviously, and the percentage of adipocyte area was increased significantly. Compared with radiation group, 1,25-(OH)2D3 reduced the decrease rate of body weight (t=-2.23,-2.34,P<0.05), partly recovered the number of white blood cells at 4 or 8 d after irradiation(t=-4.99,-4.46,P<0.05), and reduced the proportion of adipocyte area (t=-3.75,-2.10, P<0.05). With immunohistochemistrical assay, it was found that 1,25-(OH)2D3 inhibited adipogenesis by reducing the expression of PPARγ. Conclusions 1,25-(OH)2D3 decreases radiation-induced adipogenesis and hence protects the bone marrow microenvironment from radiation damage. |
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