| ZHANG Li-li,LIU Xi-guang,ZHAO Ru-sen,SONG Hao,ZHANG Hong-jun,WANG Yu-kun,LI Jing-dong,LI Wei,LIANG Ye,WANG Xiang.Radiosensitive effect of TRAIL on esophageal cancer cell line-Eca109[J].Chinese Journal of Radiological Medicine and Protection,2013,33(3):278-281 |
| Radiosensitive effect of TRAIL on esophageal cancer cell line-Eca109 |
| Received:October 19, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.015 |
| KeyWords:TRAIL Esophageal cancer cell Radiosensitivity Apoptosis G2/M Arrest |
| FundProject: |
| Author Name | Affiliation | E-mail | | ZHANG Li-li | Oncology Department, Affiliated Hospital of Medical College Qingdao University, Qingdao 266003, China | | | LIU Xi-guang | Oncology Department, Affiliated Hospital of Medical College Qingdao University, Qingdao 266003, China | liuxiguang1962@yahoo.com.cn | | ZHAO Ru-sen | 临淄区人民医院肿瘤科 | | | SONG Hao | Oncology Department, Affiliated Hospital of Medical College Qingdao University, Qingdao 266003, China | | | ZHANG Hong-jun | Oncology Department, Affiliated Hospital of Medical College Qingdao University, Qingdao 266003, China | | | WANG Yu-kun | Oncology Department, Affiliated Hospital of Medical College Qingdao University, Qingdao 266003, China | | | LI Jing-dong | 临沂市人民医院肿瘤科 | | | LI Wei | 266003 青岛大学医学院附属医院肿瘤放射治疗部 | | | LIANG Ye | 266003 青岛大学医学院附属医院 中心实验室 | | | WANG Xiang | 266003 青岛大学医学院附属医院肿瘤放射治疗部 | |
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| Abstract:: |
| Objective To study the radiosensitive effect of Apo-2 ligand (Apo2L),known as tumor necrosis factor-related apoptosis inducing ligand (TRAIL), on esophageal cancer cell line-Eca109 in vitro.Methods MTT assay and clonogenic assay were performed to evaluate the cytotoxicity and radiosensitization of TRAIL, respectively. Cell apoptosis and cell cycle distribution were measured by flow cytometry (FCM). Results TRAIL inhibited cell growth in a dose-dependent manner and its 50% inhibition concentration(IC50) was 637 μg/ml. The concentration 200 μg/ml TRAIL could enhance cell radiosensitivity with a SER of 1.219. After 2, 4, 6, 8 Gy X-ray irradiation, the incidence of apoptosis in TRAIL-treated cells was higher than that of irradiation alone groups(F=16.97, 76.65, 92.86, 209.66,P<0.05). The percentage of cells in G2/M phase of TRAIL-treated groups was also higher than that of irradiation alone groups(F=9.40, 84.99, 87.61, 2025.85,P<0.05). Conclusions TRAIL could inhibit cell growth, induce apoptosis and G2/M arrest, and had radiosensitization effect on Eca-109 cells. |
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