| FAN Li,CHEN De-qing,LIU Qing-jie,FENG Jiang-bin,LU Xue,ZHAO Hua,LIU Jian-xiang.Radioprotection effect of lipoic acid amino acid salts on L20 and AHH-1 cells[J].Chinese Journal of Radiological Medicine and Protection,2013,33(3):269-272 |
| Radioprotection effect of lipoic acid amino acid salts on L20 and AHH-1 cells |
| Received:January 25, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.013 |
| KeyWords:Lipoic acid amino acid salt Lipoic acid arginine salt ESR CCK-8 Radioprotection |
| FundProject:卫生行业科研专项(201002009);中国疾病预防控制中心青年科研基金(2009A203) |
| Author Name | Affiliation | E-mail | | FAN Li | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | CHEN De-qing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Qing-jie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | FENG Jiang-bin | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LU Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | ZHAO Hua | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Jian-xiang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | jxlamy@gmail.com |
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| Abstract:: |
| Objective For developing safe and effective anti-radiation new drugs, the effects of different lipoic acid amino acid salts on radiosensitivity were investigated.Methods The free radical scavenging ability of the above salts was evaluated in Fenton system. CCK-8 assay and flow cytometry assay were performed to evaluate the survival rate of L02 and apoptosis of AHH-1 after γ-ray irradiation, respectively. Results The lipoic acid arginine salt had the best ability of scavenging free radicals in Fenton system with an IC50 of 8.40 μmol/ml. The survival assay showed that lipoic acid amino acid salts had better stability and equal ability in radioprotection (P>0.05) compared with lipoic acid. The apoptosis assay indicated that all lipoic acid amino acid salts could inhibit radiation-induced apoptosis, where lipoic acid arginine salt was more effective (t=-6.67, P<0.01). Conclusions Lipoic acid arginine salt has good radioprotection effect on L02 and AHH-1 cells by scavenging free radicals. |
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