| PIAO Chun-nan,LIU Jian-xiang,GAO Gang,CHEN Xue,RUAN Jian-lei,SU Xu.Micronucleated reticulocytes in the peripheral blood of mice exposed to 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2013,33(3):242-244 |
| Micronucleated reticulocytes in the peripheral blood of mice exposed to 60Co γ-rays |
| Received:January 24, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.006 |
| KeyWords:Radiation Bio-dosimeter Micronucleated reticulocytes Flow cytometry |
| FundProject:卫生行业科研专项(201002009) |
| Author Name | Affiliation | E-mail | | PIAO Chun-nan | Key laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Jian-xiang | Key laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | GAO Gang | Key laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | CHEN Xue | Key laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | RUAN Jian-lei | Key laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | SU Xu | Key laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To detect the changes of the percentage of micronucleated reticulocytes(MN-RET)in the peripheral blood of mice exposed to 60Co γ-rays, in order to provide evidence for a new biomarker of radiation biodosimetry.Methods ICR mice were irradiated in whole body with 0, 0.5, 1, 2, 4 and 8 Gy at a dose rate of 0.24 Gy/min. Peripheral blood was collected for MN-RET assay using a flow cytometry. Results The percentage of peripheral MN-RET increased steadily with irradiation doses up to 2 Gy and then had a downtrend beyond 2 Gy. The changes of MN-RET observed with a microscope were consistent with the results from flow cytometry. The dose response of the MN-RET fitted to a lineal model (R2=0.9063), and the MN-RET at 2 Gy was significantly higher than that of nonirradiated control (t=-2.856,P<0.05).Conclusion Percentage of peripheral MN-RET could be an early, rapid and high-throughput radiation bio-dosimeter in certain range of doses. |
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