| FU Yue,DU Li-qing,WANG Yan,XU Chang,CAO Jia,WANG Qin,LIU Jian-xiang,LIU Qiang,FAN Fei-yue,FAN Sai-jun,SU Xu.Effect of resveratrol on the expression of IL-1β in mesenchymal stem cells exposed to ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2013,33(3):239-241 |
| Effect of resveratrol on the expression of IL-1β in mesenchymal stem cells exposed to ionizing radiation |
| Received:February 04, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.005 |
| KeyWords:Resveratrol Mesenchymal stem cells Ionizing radiation IL-1β |
| FundProject:国家自然科学基金(31200634, 31170804, 31240052);卫生行业科研专项(201002009);天津市自然科学基金(11ZCGYSY02400, 12JCYBJC15300, 12JCYBJC32900) |
| Author Name | Affiliation | E-mail | | FU Yue | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | DU Li-qing | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | WANG Yan | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | XU Chang | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | CAO Jia | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | WANG Qin | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | LIU Jian-xiang | 中国疾病预防控制中心辐射防护与核安全医学所 | | | LIU Qiang | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | liuqiang@irm-cams.ac.cn | | FAN Fei-yue | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | FAN Sai-jun | Department of Radiation Hazard Evaluation, Institute of Radiation Medicine of Chinese Academy of Medical Science and Peking Union Medical College, Tianjin Key Lab of Molecular Nuclear Medicine, Tianjin 300192, China | | | SU Xu | 中国疾病预防控制中心辐射防护与核安全医学所 | |
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| Abstract:: |
| Objective To investigate the effect of resveratrol on IL-1β production in mesenchymal stem cells (MSCs) exposed to radiation and evaluate the radioprotective effect of resveratrol. Methods MSCs were divided into non-treated control group, irradiated group and resveratrol-treated group where resveratrol with different dosage of 50, 100 and 200 μmol/L was administrated to MSCs before irradiation. The expressions of IL-1β mRNA and protein were detected using real time PCR, ELISA, and Western blot, respectively. Results Compared to the irradiation group, pretreatment of cell with resveratrol reduced the external secretion of IL-1β (t=83.34, 24.48, 12.52, P<0.05) as well as the intracellular expression of IL-1β protein and mRNA (t=8.70,14.77,13.90, P<0.05).Conclusions Resveratrol could inhibit the induction of inflammatory factor IL-1β in the irradiated MSCs. |
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