| ZHU Xiang,CAO Bao-shan,ZHANG Zhao-hui,MA Li-wen,SU Xu,LIANG Li.Roel of Lys63-linked TAK1 poly-ubiquitination at lysine 158 in radiation-induced NF-κB activity[J].Chinese Journal of Radiological Medicine and Protection,2013,33(3):235-238 |
| Roel of Lys63-linked TAK1 poly-ubiquitination at lysine 158 in radiation-induced NF-κB activity |
| Received:February 19, 2013 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.03.004 |
| KeyWords:Radiation effect Transforming growth factor-β-activating kinase 1 Polyubiquitination Nuclear factor-kappa B Signaling pathway |
| FundProject:卫生行业科研专项(201002009) |
| Author Name | Affiliation | E-mail | | ZHU Xiang | 100191, 北京大学第三医院病理科 | | | CAO Bao-shan | Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing 100191, China | | | ZHANG Zhao-hui | Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing 100191, China | | | MA Li-wen | Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing 100191, China | | | SU Xu | 中国疾病预防控制中心辐射防护与核安全医学所 | | | LIANG Li | 100191, 北京大学第三医院病理科 | liang.dr@163.com |
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| Abstract:: |
| Objective To study the role of the transforming growth factor-β-activating kinase 1 (TAK1) poly-ubiquitination in radiation-induced NF-κB activation.Methods FLAG-TAK1 and HA-Ub-K63 plasmids were transiently transfected into HEK-293T cells. The radiation effect on TAK1 ubiquitination was detected by immunoprecipitation and Western blot, which was further confirmed in FLAG-TAK1 HeLa stable cell line. Results At 1 h after irradiation, Lys 63-link TAK1 ubiquitination was induced in the HEK-293T cells with plasmids transfection. This result was further confirmed by using a FLAG-TAK1 stably expressed HeLa cell line. Radiation-induced IKKs and p38 phosphorylation were greatly impaired in MEFs reconstituted with TAK1 K158R mutant compared to ones with wild-type TAK1. Conclusions Lys63-linked TAK1 poly-ubiquitination at Lys-58 plays a key role in the process of radiation-induced NF-κB activation. |
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