| WU Guang-yin,HOU Pan-chang,WANG Wei,et al.Radioresistance change and the mechanism of human esophaged cancer EC9706 cells in hypoxia[J].Chinese Journal of Radiological Medicine and Protection,2013,33(2):138-141 |
| Radioresistance change and the mechanism of human esophaged cancer EC9706 cells in hypoxia |
| Received:November 01, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.02.007 |
| KeyWords:Esophageal cancer Hypoxia-inducible factor-1 alpha (HIF-1α) Vascular endothelial growth factor-A (VEGF-A) Vascular endothelial growth factor-D (VEGF-D) |
| FundProject:河南省科技厅科技基础与前沿技术研究计划项目(112300410296) |
| Author Name | Affiliation | E-mail | | WU Guang-yin | Department of Oncology Radiotherapy, Henan Provincial People’s Hospital, Zhengzhou 450003, China | | | HOU Pan-chang | Department of Oncology Radiotherapy, Henan Provincial People’s Hospital, Zhengzhou 450003, China | | | WANG Wei | Department of Oncology Radiotherapy, Henan Provincial People’s Hospital, Zhengzhou 450003, China | | | LUO Jian-chao | Department of Oncology Radiotherapy, Henan Provincial People’s Hospital, Zhengzhou 450003, China | | | ZHU Shao-cheng | Department of Oncology Radiotherapy, Henan Provincial People’s Hospital, Zhengzhou 450003, China | zsc2686@yahoo.com.cn |
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| Abstract:: |
| Objective To investigate the expression of hypoxia-inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor-A (VEGF-A) and vascular endothelial growth factor-D (VEGF-D) in hypoxic environment as well as the relationship between HIF-1α and VEGF-D. Methods Human esophageal cancer cell line EC9706 was cultured under hypoxia environment for 6, 12 and 24 h, the cell radiosensitivity was evaluated by survival curve. HIF-1α siRNA was constructed and transfected into human EC9706 cells. Protein expressions of HIF-1α, VEGF-A and VEGF-D were analyzed by Western blot before and after RNA interference. Results EC9706 cells under hypoxia showed radioresistance with a SF2 of 0.62 higher than that of normoxic cells of 0.43. Moreover, the protein expressions of HIF-1α, VEGF-A and VEGF-D were all increased (F=205.24, 227.88, 130.55,P<0.05) due to hypoxia treatment. On the contrary, after HIF-1α siRNA transfer, the protein expressions of HIF-1α, VEGF-A and VEGF-D in EC9706 cells were not influenced by hypoxia treatment.Conclusions EC9706 cells in hypoxic environment was radioresistance, and the upexpressions of HIF1α, VEGF-A and VEGF-D may be involved. |
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