| SUN Bao-sheng,ZUO Ya-fang,TIAN Qi,et al.The role of miR-92a and BCL11b in radiation induced thymic lymphoma in BALB/c mice[J].Chinese Journal of Radiological Medicine and Protection,2013,33(2):135-137 |
| The role of miR-92a and BCL11b in radiation induced thymic lymphoma in BALB/c mice |
| Received:August 10, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.02.006 |
| KeyWords:Ionizing radiation Thymic lymphoma BCL11b miR-92a |
| FundProject:吉林省自然科学基金(201111211) |
| Author Name | Affiliation | E-mail | | SUN Bao-sheng | Department of Oncology, Tumor Hospital of Jilin Province, Changchun 130012, China | | | ZUO Ya-fang | Department of Oncology, Tumor Hospital of Jilin Province, Changchun 130012, China | | | TIAN Qi | Department of Oncology, Tumor Hospital of Jilin Province, Changchun 130012, China | | | OU Jian | Department of Oncology, Tumor Hospital of Jilin Province, Changchun 130012, China | | | ZHANG Guo-sheng | Department of Oncology, Tumor Hospital of Jilin Province, Changchun 130012, China | | | LIU Jiang-shan | Department of Oncology, Tumor Hospital of Jilin Province, Changchun 130012, China | | | LIU Bo-yu | Department of Oncology, Tumor Hospital of Jilin Province, Changchun 130012, China | | | LIU Shi-Xin | Department of Oncology, Tumor Hospital of Jilin Province, Changchun 130012, China | liushixin1964@sina.com |
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| Abstract:: |
| Objective To investigate the expression of miR-92a and BCL11b gene in radiation-induced thymic lymphoma in BALB/c mice, and the regulation of BCL11b by miR-92a. Methods BALB/c mice were irradiated with 1.75 Gy gamma rays with a dose rate of 0.382 Gy/min, 4 weeks in a row and once per week. 6 months after radiation, the incidence of the thymic lymphoma induced by ionizing radiation was checked and the mice were sacrificed. The thymuses of 6 typical mice with thymic lymphoma in the irradiated group and 6 mice in control group were taken for further detection. Real-time PCR were used to detect miR-92a expression. Western blot and FACS were employed to measure protein expression of BCL11b. The miR-92a sequence was inserted into pcDNA-DEST-47 plasmid to construct the eukaryotic expression vector (pcDNA-DEST-miR-92a) and to construct the BCL11b gene 3'UTR luciferase reporter plasmid (psiCHECK 2-BCL11b) at the same time. They were used to transfect EL-4 cells together for analysis of the regulation of miR-92a on the expression of BCL11b. Results The incidence of the thymic lymphoma induced by ionizing radiation was 58.51%. It was found that expression of the tumor suppressor gene BCL11b was down-regulated and expression of miR-92a was up-regulated in radiation induced thymic lymphoma tissue samples. There was an inverse correlation between BCL11b protein and miR-92a expression level among different tissue samples (r=-0.827, P<0.05). The expression of luciferase in the group transfected with pcDNA-DEST-miR-92a and psiCHECK 2-BCL11b plasmids was significantly lower than that in the group transfected with empty plasmid and BCL11b gene (t=3.42,P＜0.05). Conclusions miR-92a maybe participate in radiation-induced carcinogenesis and regulate the expression of BCL11b gene. |
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