| JIANG Mao-qing,CHEN Hao-jun,DOU Xiao-feng,CHEN Xin-jia,LIN Qin,FAN Wen-bo,PENG Tian-xing,YAN He-ping,SUN Long,WU Hua.Using 18F-FLT and 18F-FDG to evaluate the ultra-early biological response of esophageal carcinoma cells after irradiation[J].Chinese Journal of Radiological Medicine and Protection,2013,33(2):131-134 |
| Using 18F-FLT and 18F-FDG to evaluate the ultra-early biological response of esophageal carcinoma cells after irradiation |
| Received:October 24, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.02.005 |
| KeyWords:18F-FDG 18F-FLT Esophageal carcinoma cells Biological response |
| FundProject:福建省自然科学基金(2008-59-11);厦门市科技计划指导性项目(3502720077056) |
| Author Name | Affiliation | E-mail | | JIANG Mao-qing | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | CHEN Hao-jun | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | DOU Xiao-feng | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | CHEN Xin-jia | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | LIN Qin | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | FAN Wen-bo | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | PENG Tian-xing | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | YAN He-ping | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | SUN Long | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | | | WU Hua | The First Affiliated Hospital of Xiamen University, Xiamen 361001, China | wuhua1025@163.com |
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| Abstract:: |
| Objective To compare the difference of 3'-deoxy-3'-18F-fluorothymidine (18F-FLT) and 2'-deoxy-2'-18F-fluoro-D-glucose (18F-FDG) in the ultra-early biological response of esophageal carcinoma cells after irradiation. Methods After 2, 4 and 8 h of irradiation with different doses (5, 10, 15 Gy), the uptake ratio of 18F-FDG and 18F-FLT, survival, and ATP level of esophageal carcinoma cells were detected. Results After 2 and 4 h of 5 Gy irradiation, the cellular 18F-FDG uptake ratio was decreased by 9.45% and 16.4%. After 2 h of 15 Gy irradiation, this uptake ratio was increased by 26.5% (t=3.04, P<0.05). But the 18F-FDG uptake ratios of the other experimental groups were decreased remarkably (F=25.75, P<0.05). On the other side, after 2 h of 5 Gy irradiation, the 18F-FLT uptake ratio had no statistically difference [(3.65±0.17)% vs (4.00±0.17)%, P>0.05], and 18F-FLT uptake ratios of the other groups were decreased notably (F=33.93, P<0.05). The relative survival of esophageal carcinoma cells after 2, 4 and 8 h of irradiation with different doses (5, 10, 15 Gy) has no significant difference. Line regression analysis indicated a significant correlation between 18F-FLT and ATP levels (r=0.887, P<0.05), whereas a less correlation was found between 18F-FDG and ATP levels (r=0.622, P>0.05). Conclusions Both 18F-FDG and 18F-FLT can evaluate the ultra-early irradiation response of esophageal carcinoma cells, but 18F-FLT may be more specific than 18F-FDG in monitoring the ultra-early irradiation response. |
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