| CHEN Xue,GAO Gang,RUAN Jian-lei,et al.Effects of exogenetic miR-34a on radiosensitivity of H1299 cells[J].Chinese Journal of Radiological Medicine and Protection,2013,33(2):128-130 |
| Effects of exogenetic miR-34a on radiosensitivity of H1299 cells |
| Received:November 20, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.02.004 |
| KeyWords:miR-34a H1299 cells Radiosensitivity Apoptosis Cell cycle |
| FundProject:国家自然科学基金(31100606);卫生行业科研专项(201002009) |
| Author Name | Affiliation | E-mail | | CHEN Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | GAO Gang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | RUAN Jian-lei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Jian-xiang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | jxliu@nirp.cn |
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| Abstract:: |
| Objective To investigate the influences of miR-34a on the radiosensitivity of H1299 cells. Methods CCK-8 kit was used to examine the viability of H1299 cells which were exposed to different doses (0, 2, 4, 6 and 8 Gy) of 60Co γ-rays after transient transfection of pre-miR-34a. Apoptosis rate and cell cycle were measured with flow cytometry. The expression levels of miR-34a target genes, bcl-2, bax, CDK4, CDK6 and cyclinD1 were analyzed by real-time PCR. Results Compared to the control group of negative transfection, the cell viability in pre-miR-34a transfection group decreased significantly after irradiation at 0, 2, 4, 6, 8 Gy (t=-2.39, -3.12, -4.98, -4.03, -3.06, P<0.05) in a dose-dependent manner. After being irradiated with 6 Gy γ-rays, the apoptotic rate in pre-miR-34a transfection group was significantly increased (t=7.06, P<0.05) together with an accumulation of G0/G1 phase (t=3.94, P<0.05) and a reduction of S phase (t=6.23, P<0.05). The gene expression levels of bcl-2, CDK4 and CDK6 in pre-miR-34a transfection group were respectively decreased (t=3.39, 12.88, 6.21, P<0.05) of negative control. cyclinD1 was also decreased but no significance, while bax was increased to 1.94 times of negative control (t=-4.35, P<0.05) together with a decrease of bcl-2/bax. Conclusions miR-34a could promote cell apoptosis, induce G0/G1 phase accumulation, suppress cell activity, and in turn increase the radiosensitivity of H1299 cells. |
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