| XIONG Shan-shan,LI Ruo-xi,DU Li,et al.Changes of fibrocytes and related Th1/Th2 cytokines in mice with radiation-induced pulmonary injury[J].Chinese Journal of Radiological Medicine and Protection,2013,33(2):124-127 |
| Changes of fibrocytes and related Th1/Th2 cytokines in mice with radiation-induced pulmonary injury |
| Received:August 14, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.02.003 |
| KeyWords:Radiation-induced pulmonary injury Fibrocytes Cytokines of MCP-1 Cytokines of MIP-1α Cytokines of Th1/Th2 |
| FundProject:国家自然科学基金(81001219,30900381) |
| Author Name | Affiliation | E-mail | | XIONG Shan-shan | Institute of Radiation Medicine, AMMS, Beijing 100850, China | | | LI Ruo-xi | Institute of Radiation Medicine, AMMS, Beijing 100850, China | | | DU Li | Institute of Radiation Medicine, AMMS, Beijing 100850, China | | | XIAO Feng-jun | Institute of Radiation Medicine, AMMS, Beijing 100850, China | | | XU Long | Institute of Radiation Medicine, AMMS, Beijing 100850, China | | | LIU Kun-lu | Institute of Radiation Medicine, AMMS, Beijing 100850, China | | | YANG Zhi-hua | Institute of Radiation Medicine, AMMS, Beijing 100850, China | | | ZHU Mao-xiang | Institute of Radiation Medicine, AMMS, Beijing 100850, China | | | PAN Xiu-jie | Institute of Radiation Medicine, AMMS, Beijing 100850, China | aaronpancn@126.com |
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| Abstract:: |
| Objective To observe the changes of fibrocytes and Th1/Th2 cytokines in the lung of irradiated mice and explore its role in radiation-induced pulmonary injury. Methods The whole thorax of mice were irradiated with single 25 Gy of 60Co γ-rays in order to establish a model of pulmonary injury. The pathologic changes of lung were determined by Masson three-color stain and Hematoxylin and eosin stain. The concentration of collagenⅠwas assayed by microplate spectrophotometer. The number of fibrocytes in lung and the levels of MCP-1, MIP-1α and Th1/Th2 cytokines in BAL were measured with flow cytometry and liquid suspension array, respectively. Results Irradiated mice suffered with weight loss from 14 d to 3 month after irradiation (F=7.19, 40.62, 58.70, P<0.05). Pathological evidence demonstrated that the collagen fibers hyperplasia occurred at 1 month and aggravated at 3 month. Concentration of collagenⅠ(Z=-2.470, -2.236, P<0.05)increased at 1 and 3 month post-irradiation. There were significant increase in the number of fibrocytes (Z=-2.19, -2.64, -2.64,P<0.05) in pulmonary interstitium and the levels of MCP-1 (Z=-2.35, -2.37, -2.34, P<0.05) and MIP-1α (Z=-2.43, -2.35, -2.35, P<0.05) in BAL from 14 d to 3 month. The levels of IFN-γ increased at 1 month (Z=-2.34 P<0.05) and the expression of IL-12 decreased at 3 and 7 d (Z=-2.31, -2.30, P<0.05) while increased at 1 and 3 month (Z=-2.32,-2.12, P<0.05).IL-4 level were up from 7 d to 1 month (Z=-2.31, -2.35, -2.32, P<0.05), and IL-10 level went up on 3 d, at 1 month and 3 month post-irradiation (Z=-2.33, -2.37, -2.34, P<0.05). Conclusions Fibrocytes are the important target of radiation-induced pulmonary injury. MCP-1and MIP-1α lead to fibrocytes recruitment and Th1/Th2 cytokines may be involved in this process. |
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