| CHEN Ting-ting,ZHANG Xi-zhi,WANG Bu-hai,et al.The mechanism of Deinococcus radiodurans pprI gene in enhancing mice radioresistance to γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2013,33(2):119-123 |
| The mechanism of Deinococcus radiodurans pprI gene in enhancing mice radioresistance to γ-rays |
| Received:September 25, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.02.002 |
| KeyWords:Deinococcus radiodurans pprI gene in vivo electroporation Radiation injury |
| FundProject:国家自然科学基金(81072236);国防基础科研资助项目(A3820060138);江苏高校优势学科建设工程资助项目 |
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| Abstract:: |
| Objective To investigate the mechanism of Deinococcus radiodurans pprI gene in enhancing mice radioresistance to γ-rays by transfecting it in vivo.Methods The male Kunming mice were randomly divided into control group, irradiated group, pCMV-HA transfected group and pCMV-HA-pprI transfected group. The pCMV-HA-pprI plasmid contained pprI gene was injected into the muscle of mice which were exposed to total 6 Gy of γ-ray irradiation. After injection, the in vivo gene electroporation technology was used to transfect the pprI gene into the cells, and Western blot was used to identify the PprI protein, mammalian homolog protein Rad51 corresponding to recA gene downstream of pprI, and protein Rad52.Results In the muscle of the mice of transfected pCMV-HA-pprI group, the protein PprI expressed significantly at 1 d post-irradiation, but there was no expression of pprI gene 7 d post-irradiation and in other groups. In the mice of transfected with pCMV-HA-pprI, the expression of Rad51 protein was significantly increased in the lungs at 1, 7 and 14 d post-irradiation, and significantly increased in the liver at 1 and 28 d post-irradiation and increased in the kidneys at 1 and 14 d post-irradition. However, there was no obvious change of Rad52 protein expression in the lungs and livers of mice in all groups.Conlusions The prokaryotic gene pprI could act on the mammalian homologisation analogues rad51 gene downstream of recA gene and then increase the expression level of protein Rad51 which results in the enhancement of radioresistance. |
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