| ZHU Ya-qun,LIAO Yan-sen,ZHANG Bin,DENG Sheng-ming,WU Yong-you,TIAN Ye.Investigation of early radiation response by evaluating the capability of 18F-FLT uptake in human colorectal cancer HCT116 cells[J].Chinese Journal of Radiological Medicine and Protection,2013,33(1):27-31 |
| Investigation of early radiation response by evaluating the capability of 18F-FLT uptake in human colorectal cancer HCT116 cells |
| Received:August 04, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.01.007 |
| KeyWords:18F-FLT Uptake Colorectal cancer Radiotherapy |
| FundProject:江苏省"十二五"临床医学重点学科资助;苏州市社会发展项目(SYSD2011095);苏州市科技发展计划项目(SYS201031) |
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| Abstract:: |
| Objective To evaluate the capability of 18F-FLT uptake and investigate the early radiation response of human colorectal cancer cells HCT116 exposed to 6 MV X-rays.Methods 3.7 kBq 18F-FLT was added to HCT116 cells with different cell numbers (1.0×105-1.5×106) and cultured with different times (36, 60, 84 h). The 18F-FLT uptake rate was measured with a γ-counter after exposed to different does of 6 MV X-rays (0, 2, 4, 6, 8 Gy) after 24, 48, and 72 h of irradiation. Then the cell uptake inhibition rate, cell proliferation, and cell cycle phase were measured.Results The uptake rate of 18F-FLT in HCT116 was (18.97±1.16)%. The 18F-FLT uptake inhibition rates at 24 h after different does of irradiation (2, 4, 6, 8 Gy) were (32.10±0.02)%, (54.46±0.04)%, (62.74±0.04)%, and (65.81±4.81)%, respectively, which was positively correlated with radiation dose.Conclusions The 18F-FLT uptake rate of human colorectal cancer HCT116 cells could be used to evaluate the early radiation response. |
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