| LIU Xing,NIE Ji-hua,CHEN Zhi-hai,et al.DNA damage in HBE cells induced by radon in combination with sodium arsenite[J].Chinese Journal of Radiological Medicine and Protection,2013,33(1):19-22 |
| DNA damage in HBE cells induced by radon in combination with sodium arsenite |
| Received:June 02, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.01.005 |
| KeyWords:Radon Sodium arsenite Combination effect DNA damage |
| FundProject:国家自然科学基金重大国际(地区)合作研究项目(81020108028);江苏省高校青年自然基金(12KJB33005) |
| Author Name | Affiliation | E-mail | | LIU Xing | School of Public Health, Soochow University, Suzhou 215123, China | | | NIE Ji-hua | School of Public Health, Soochow University, Suzhou 215123, China | | | CHEN Zhi-hai | School of Public Health, Soochow University, Suzhou 215123, China | | | JIN Huan-yu | School of Public Health, Soochow University, Suzhou 215123, China | | | CHEN Qiu | 215123 苏州大学放射医学与防护学院 | | | ZHOU Xin-wen | 215123 苏州大学放射医学与防护学院 | | | JIAO Yang | 215123 苏州大学放射医学与防护学院 | | | TONG Jian | School of Public Health, Soochow University, Suzhou 215123, China | tongj@suda.edu.cn | | AN Yan | School of Public Health, Soochow University, Suzhou 215123, China | |
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| Abstract:: |
| Objective To study DNA damage effect of radon in combination with sodium arsenite on HBE cells.Methods The HBE cells were exposed to various concentrations of radon (20 kBq/m3, 0-75 min), sodium arsenite (0-50 mmol/L) and their combinations. Cell viability was tested by cell counting kit-8 (CCK-8). The combination index (CI) was calculated by different time exposed radon combined with different concentrations sodium arsenite. Single cell gel electrophoresis (SCGE) was applied for quantitative analysis of DNA damage. Results Radon with or without sodium arsenite inhibited the growth of HBE cells. Computed using median-effect principle, CI=0.41 indicated that radon and sodium arsenite had synergistic effect. Radon and sodium arsenite both could significantly increase tail DNA, tail moment and olive tail moment of HBE cells in SCGE compared with control (t=12.90-23.61,8.81-21.39,18.46-38.97,P<0.05; t=18.46-39.67,17.41-27.42,28.03-46.32,P<0.05). There was a dose-response relationship between radon level, sodium arsenite and DNA damage. The results of factorial analysis showed that there was a combination effect of DNA damage induced by radon and sodium arsenite (Ftail DNA%=76.07,P<0.01;Ftail moment=290.51,P<0.01;FOliver tail moment=273.46,P<0.01). Conclusions There is a synergistic effect of DNA damage in HBE cells irradiated by radon in combination with sodium arsenite. |
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