| LIU Lan-tao,LIU Jian-xiang,GAO Gang,RUAN Jian-lei,PAN Yan,PIAO Chun-nan,CHEN Xue,SU Xu.Changes of lymphocytes and regulatory T cell subset of mice influenced by 60Co γ-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2013,33(1):14-18 |
| Changes of lymphocytes and regulatory T cell subset of mice influenced by 60Co γ-ray irradiation |
| Received:September 24, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.01.004 |
| KeyWords:Ionizing radiation Regulatory T cell Phenotype |
| FundProject:卫生行业科研专项(201002009) |
| Author Name | Affiliation | E-mail | | LIU Lan-tao | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Jian-xiang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | GAO Gang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | RUAN Jian-lei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | PAN Yan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | PIAO Chun-nan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | CHEN Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | SU Xu | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To analyze the influence of ionizing radiation on the lymphocytes and its regulatory T cells in mice. Methods Mice were administered with whole body irradiation of γ-rays at different doses, and lymphocytes were separated from thymus and spleen, then the number of total cells were counted and the percentages of CD4+T and CD4+FOXP3+CD25+ Treg lymphocytes were analyzed by using FACS. Results The lymphocyte numbers in thymus and spleen decreased in dose-dependent manner and reached to the minimum at 4 d after irradiation (F=118.08,144.01,P<0.05). Exposure to higher dose(more than 1 Gy) decreased Treg number time-dependently in thymus, however increased it in spleen. On the contrary, exposure to lower dose(less than 0.75 Gy) increased Treg number in thymus. Besides, the percentage of Treg cells increased dose-dependently(in thymus, F=5.16,89.44,3.01,P<0.05;in spleen, F=52.02,32.13,27.45,P<0.05). Conclusions The radiation responses of lymphocytes and their Treg subpopulation vary with the different doses. Treg cells are resistant to high dose irradiation, however, their differentiation could be induced by low dose irradiation. In addition, the different time-dependent responses of lymphocytes and their subpopulation to ionizing radiation indicate the difference of lymphocyte maturation, differentiation and emigration. |
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