| SUN Ting,DING Da-dong,LI Bin,et al.Apoptosis of human melanoma cells induced by boron neutron capture therapy[J].Chinese Journal of Radiological Medicine and Protection,2013,33(1):10-13 |
| Apoptosis of human melanoma cells induced by boron neutron capture therapy |
| Received:May 15, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.01.003 |
| KeyWords:Boron neutron capture therapy Boronophenylalanine In-hospital neutron irradiator Apoptosis |
| FundProject:国家自然科学基金(81207142);江苏省医学领军人才与创新团队项目(K201106) |
| Author Name | Affiliation | E-mail | | SUN Ting | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | DING Da-dong | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | LI Bin | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | CHEN Gui-lin | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | WEI Yong-xin | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | XIE Xue-shun | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | YANG Tian-quan | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | WU Ting-feng | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | ZHOU You-xin | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | Zhouyxyq2008@sohu.com | | DU Zi-wei | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China | |
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| Abstract:: |
| Objective To study the effect and underlying mechanism of boron neutron capture therapy (BNCT) on human melanoma cells. Methods The situation of boronophenylalanine (BPA) uptake of human melanoma cells A375 was detected and then the boron-10 (10B) enriched cells were irradiated by an in-hospital neutron irradiator (IHNI-1). The radiation sensitivity was measured using clonogenic survival assay, the proliferation was examined by MTT assay, apoptosis was determined using flow cytometry, and the protein expression of cytochrome C in cytosol and activation of caspase-9 was detected by Western blot.Results 10B concentration in A375 cells approached to (2.884±0.148) μg/107 cells after 24 h culture with BPA, which met the requirement of BNCT. At 2.1 min after neutron radiation, the survival fraction of BNCT group was decreased to 58% of control (t=2.964,P<0.05). At 24 h after BNCT, the cell viability was decreased to 83% of control (t=3.286, P<0.05), the apoptosis ratio was (55.2±7.9)%(t=9.754, P<0.05), and the protein level of cytochrome C in cytosol and the activativity of caspase-9 were also enhanced (t=7.625,8.307, P<0.05).Conclusions Human melanoma cells can be killed by BNCT due to apoptosis through a mitochondrial pathway. |
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