| CHONG Yu,XU Jia-ying,YANG Yang,JIAO Yang,ZHANG Zhi-jun,FAN Sai-jun.Effect of sanguinarine on growth and radiosensitivity of human glioma A172 cells[J].Chinese Journal of Radiological Medicine and Protection,2013,33(1):1-5 |
| Effect of sanguinarine on growth and radiosensitivity of human glioma A172 cells |
| Received:October 21, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.01.001 |
| KeyWords:Sanguinarine Glioma Apoptosis Reactive oxygen species Radiosensitivity |
| FundProject:国家自然科学基金(81071906;81172127) |
| Author Name | Affiliation | E-mail | | CHONG Yu | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | XU Jia-ying | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | YANG Yang | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | JIAO Yang | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | ZHANG Zhi-jun | 中国科学院苏州纳米技术与纳米仿生研究所 | | | FAN Sai-jun | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | sjfan@suda.edu.cn |
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| Abstract:: |
| Objective To study the effect of sanguinarine on growth and radiosensitivity of human glioma A172 cells. Methods MTT assay was used to evaluate cell growth. Cell cycle analysis and reactive oxygen species(ROS) burst were performed by flow cytometry assay. Annexin V/PI assay was used to detect cell apoptosis. Colony formation assay was used to detect the influence of sanguinarine on cell radiosensitivity. Results Exposure of A172 cells to sanguinarine led to dose- and time- dependent cytotoxicity with IC50 values of 4.8, 3.9 and 3.2 μmol/L for 12, 24 and 48 h, respectively. Furthermore, sanguinarine caused an arrest of S phase. After being treated with 5 μmol/L sanguinarine for 24 h, the ratios of early apoptosis, late apoptosis and necrosis were (60.01±3.73)%,(2.70±0.18)% and (3.93±0.76)% respectively in A172 cells compared with the untreated control (t=55.28, 8.32, 9.51, P<0.05). An increased generation of ROS was found after treatment with sanguinarine, however, NAC inhibited the effect of sanguinarine. As analyzed with multi-target click model fitting curves, the SERD0 of sanguinarine-treated cells was 1.48. Conclusions Sanguinarine inhibits the A172 cells growth via apoptosis induction and ROS burst. Moreover, sanguinarine at a non-cytotoxicity dose increases cell sensitivity to X-rays. |
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