| XU Xiao-ting,ZHOU Ju-ying,LIU Wei,LI Li,WU Qiong,QIN Song-bing,WANG Li-li.Effect of radiation on the radiosensitivity of a human malignant glioma cell line[J].Chinese Journal of Radiological Medicine and Protection,2012,32(6):602-606 |
| Effect of radiation on the radiosensitivity of a human malignant glioma cell line |
| Received:April 05, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.06.010 |
| KeyWords:Glioma Radioresistance cyclin B1 miR-21 Stat3 |
| FundProject: |
| Author Name | Affiliation | E-mail | | XU Xiao-ting | Department of Radiation Oncology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | ZHOU Ju-ying | Department of Radiation Oncology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China | Zhjuying@sohu.com | | LIU Wei | Department of Radiation Oncology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | LI Li | Department of Radiation Oncology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | WU Qiong | Department of Radiation Oncology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | QIN Song-bing | Department of Radiation Oncology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China | | | WANG Li-li | Department of Radiation Oncology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China | |
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| Abstract:: |
| Objective To observe the differences of the radio-biological characteristics between the human malignant glioma cell line SHG-44 and its progeny cells SHG-4410 Gy and to probe the underlying mechanism. Methods The SHG-4410 Gy cells were the progeny of SHG cells that had been irradiated with 10 Gy X-rays and then passaged for 15 generations. The radiosensitivity of SHG-44 and SHG-4410 Gy wre measured by clonogenic assay and the multi-target single-hit model was used to fit the survival curve. The cell cycle redistribution and apoptosis were analyzed by flow cytometry assay. Quantitative Real Time-PCR (qRT-PCR) was used to determine the relative levels of cyclin B1 mRNA and miR-21. Stat3 protein levels were detected by Western blot. Results The values of D0, Dq and SF2 of SHG-4410 Gy cells were significantly higher than those of SHG-44 cells. Flow cytometric analysis showed that there was less G2/M phase arrest in SHG-4410 Gy (F=22.21, P<0.05). Radiation-induced early apoptotic population was increased from (17.60± 0.26)% to (28.00± 0.36)% for SHG-44 cells, but increased from only (4.20± 0.30)% to (5.17± 0.65)% for SHG-4410 Gy. miR-21 in SHG-4410 Gy cells were increased by 1.44 fold of SHG-44 cells, which was associated with the increase of Stat3 protein expression. Conclusions Radioresistance is observed in the progeny of human malignant glioma cell line SHG-44 which had been irradiated with 10 Gy X-rays. The underlying mechanisms may be relative to the upregulation of cyclin B1 that acts as a key downstream gene in the signaling pathway of G2/M phase transition. In addition, the upregulation of miR-21 may be involved in the apoptosis of SHG-4410 Gy cells. |
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