| LU Liang-jie,DONG Juan-cong,ZHANG Cong,JIN Shun-zi,SHAN Yu-xing.TLR4 enhances the radiation sensitivity of tumor cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(6):583-587 |
| TLR4 enhances the radiation sensitivity of tumor cells |
| Received:May 24, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.06.006 |
| KeyWords:Ionizing radiation Toll-like receptor-4 Tumor cells Radiosensitivity |
| FundProject:国家自然科学基金(30870584) |
|
| Hits: 3504 |
| Download times: 2083 |
| Abstract:: |
| Objective To investigate the effects of TLR4 on the radiosensitivity of tumor cells. Methods The cell lines of RAW264.7, Lewis, MFC, Hepa1-6, B16, and NIH3T3 were irradiated with 5 Gy X-rays or sham-irradiated. 24 h after irradiation, the expression of TLR4 was detected by flow cytometry. According to the TKR4 level, cells were divided into three groups: without treatment, LPS stimulation and TAK242 block. CCK-8 kit and Annexin-V Apoptosis Kit were used to detect cell proliferation, apoptosis and cell cycle distribution of each group. Results After 24 h of 5 Gy ionizing radiation, TLR4 was significantly increased in Lewis cells (t=-8.68, P<0.01) but decreased in MFC cells (t=25.8, P<0.01) and had no significant changes in Hepa1-6, B16 and RAW264.7 cells. In addition, the proliferation vitality (t=57.62,-6.23, P<0.01) and survival fraction (t=13.37,19.24,P<0.01) of the Lewis and MFC cells were reduced especially for the TLR4-blocked cells, and the apoptosis rates of both Lewis (t=-167.85, P<0.01) and MFC cells (t=-26.45, P<0.01) were elevated. The percentages of G0/G1 phase and S phase Lewis cells were significant increased (t=8.68, 14.89, P<0.01) but its G2/M phase were reduced (t=-37.48, P<0.01). However, the percentages of G0/G1 phase and S phase MFC cells were obviously reduced (t=20.31, 4.48, P<0.01) and G2/M phase increased (t=-13.06, P<0.01). For both cell lines of Lewis and MFC, the cycle distribution of TAK242 and LPS groups didn't change significantly. Conclusions High expression TLR4 in the Lewis cells is related to cell proliferation and apoptosis but not cell cycle distribution, and hence TLR4 could influence the radiosensitivity of tumor cells. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
