| DONG Juan-cong,SHAN Yu-xing,SHAO Ming-long,ZHANG Cong,LU Liang-jie,JIN Shun-zi.Role of PLC/PIP2 signaling pathway in radiation-induced NRP1+Treg cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(6):561-564 |
| Role of PLC/PIP2 signaling pathway in radiation-induced NRP1+Treg cells |
| Received:May 11, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.06.001 |
| KeyWords:Ionizing radiation Neuropilin-1 Regulatory T-Lymphocytes TGF-β1 PLC/PIP2 signal pathway |
| FundProject:国家自然科学基金(30870584);国家教育部留学回国人员启动基金(20101201) |
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| Abstract:: |
| Objective To explore the role of PLC/PIP2 signal pathway in the changes of mouse thymus CD4+CD25+NRP1+ Treg and TGF-β1 after different doses of X-ray irradiation Methods 36 ICR mice were randomly divided into 6 groups according to the irradiation doses of 0, 0.5, 1.0, 2.0, 4.0 and 6.0 Gy, respectively. Flow cytometry was used to detect the expression of NRP1+ Treg, and ELISA was used to detect the expression of TGF-β1 in mouse thymocytes at 16 h post-irradiation. The EL-4 cells were irradiated by X-rays at the dose of 4.0 Gy after co-cultured with the PMA and TMB-8 for 2 hours. Flow cytometry was used to detect the expression of NRP1+ Treg, and ELISA was used to detect the changes of TGF-β1 at 48 h post-irradiation. Results The NRP1+ Treg appeared a transient decrease at both 0.5 and 1.0 Gy irradiation and reached its valley value at 1.0 Gy (t=6.96, P<0.01), then showed a dose-dependent increase and reached its peak at 6.0 Gy (t=6.70, P<0.01). The TGF-β1 level decreased after 0.5 Gy X-rays (t=12.53, P<0.01), then increased at a dose-dependent manner and reached its peak at 4.0 Gy (t=10.40-19.56, P<0.01). Compared with the sham-irradiation, NRP1+Treg was decreased significantly after PMA treatment (t=3.06, P<0.01), while it was up-regulated significantly after irradiation in the presence of PMA (t=8.27, P<0.01). TGF-β1 was reduced in the presence of PMA with or without irradiation (t=10.46-39.69, P<0.01). NRP1+Treg and TGF-β1 were increased significantly after TMB-8 treatment (t=5.53-44.26, P<0.01). Conclusions NRP1+Treg cells and TGF-β1 were up-regulated after a high dose radiation, and the PLC/PIP2 signal pathway may participate in the regulation. |
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